高效液相色谱法测定保健食品中水飞蓟素的含量

Determination of silymarin in health food by high performance liquid chromatography

目的建立高效液相色谱法（high performance liquid chromatography,HPLC）测定保健食品中水飞蓟素的含量。方法样品以甲醇为提取剂超声提取30 min,以甲醇-水（55：45,V：V）为流动相,流速为0.8 mL/min,采用色谱柱Agilent TC-C18分离,在288 nm检测波长下经HPLC检测;同时对检测波长、流动相比例、提取剂和提取时间等条件进行优化。结果水飞蓟素浓度在8.152-203.8μg/mL范围内呈良好的线性关系,相关系数为0.9999,平均回收率为100.35%,RSD值为0.95%,方法检出限为11.6 ng/g。结论该方法快速准确,可适用于保健食品中水飞蓟素含量的测定。

Objective To establish a method for the determination of silymarin in health food by high performance liquid chromatography（HPLC）. Methods Samples were extracted with methanol by ultrasonic extraction for 30 min, and separated by an Agilent TC-C18 column with methanol-water（55：45, V：V）as mobile phase at the flow rate of 0.8 m L/min, and then detected by HPLC under the detection wavelength of 288 nm. Meanwhile, the detection wavelength, mobile phase ratio, extractant and extraction time were optimized. Results Silymarin had a good linear relationship in the range of 8.152-203.8 μg/m L with the correlation coefficient of 0.9999. The average recovery and relative standard deviation（RSD） were 100.35% and 0.95% respectively. The limit of detection was 11.6 ng/g. Conclusion The method is rapid and accurate, which can be used for the determination of silymarin in health food.