大孔树脂法纯化香菇柄中多酚的工艺优化

Optimization of purification process of polyphenol from Lentinus edodes stem by macroporous resin adsorption

目的优化大孔树脂法纯化香菇柄中多酚类物质的工艺。方法以AB-8、NKA-9、D101、D280、DA201 5种大孔树脂对香菇柄中多酚的吸附率和解吸率为评价指标,通过静态吸附和解吸试验筛选最佳树脂。然后研究吸附温度、树脂质量、pH值大小、乙醇（洗脱剂）体积分数和乙醇（洗脱剂）体积5个因素对大孔树脂吸附和解吸香菇柄中多酚的影响。最后以香菇柄中多酚类物质得率为指标,采用L9（3-3）正交试验对纯化工艺进行优化。结果通过筛选发现,NKA-9大孔树脂吸附率和解吸率较高。正交试验优化获得的最佳工艺条件为：pH=3,树脂质量0.50 g,吸附温度50℃,在此条件下纯化的香菇柄中多酚得率为51.71%±0.95%,纯度也较未纯化时提高了3.71倍。结论从香菇柄中开发利用香菇多酚具有可行性,本研究为香菇多酚的纯化和制备提供了科学依据。

Objective To optimize the purification process of polyphenols from Lentinus edodes stem by macroporous resin. Methods The best resin among AB-8, NKA-9, D101, D280 and DA201 was chosen by experiments used the static adsorption and desorption rates of polyphenols from Lentinus edodes stem as indicator. Then the effects of adsorption temperature conditions, resin weight, pH conditions, eluent volume fractions and eluent volumes on adsorption and desorption rates were investigated. Finally, orthogonal experiment of L9（3~3） was used to optimize the purification process and the yield of polyphenol from Lentinus edodes stem was targeted. Results The NKA-9 resin had higher adsorption and desorption rates than other resins, and the optimum purification process conditions were as follows： pH condition was 3, the resin weight was 0.50 g and the adsorption temperature condition was 50 ℃. Under these conditions, the yield of polyphenol in Lentinus edodes stem adsorbed and desorbed by macro-porous resin was 51.71%±0.95%. The purity of polyphenols extractive was increased by 3.71 times compared with unpurified extraction. Conclusion Utilizing polyphenols from lentinus edodes stems is feasible, which can provide scientific basis for the purification and preparation of polyphenols from Lentinus edodes stems.