高效液相色谱法测定食品中的碱性橙染料

Determination of basic orange dyes in food by high performance liquid chromatography

目的采用高效液相色谱法测定食品中非法色素添加剂的碱性橙染料即碱性橙2（chrysoidine G,CDG）、碱性橙21（orange G,AOG）及碱性橙22（astrazon orange R,AOR）。方法样品经乙腈：水：氨水（60：40：0.5,V：V：V）在25℃下超声提取30 min,以0.02 mol/L的乙酸铵溶液（pH为5）和乙腈作为流动相进行梯度洗脱,采用Agilent XDB C18色谱柱进行分离,用二极管阵列检测器在449 nm波长处检测CDG,在485 nm波长处检测AOG及AOR,以外标峰面积法定量。结果 3种碱性橙染料在0.01-0.2 mg/L浓度范围内线性关系良好,方法回收率为80.0%-94.0%,相对标准偏差均小于2%（n=6）,方法的检出限为0.1 mg/kg。结论本方法操作简便准确,可适用于腐竹、干辣椒和辣椒油等不同基质样品中碱性橙的检测。

Objective To establish a method for determination of 3 kinds of basic orange dyes by high performance liquid chromatography（HPLC）, including chrysoidine G（CDG）, astrazon orange G（AOG） and astrazon orange R（AOR）. Methods Samples were extracted with ultrasonic for 30 min by acetonitrile： water： ammonium hydroxide（60：40：0.5, V：V：V） at 25 ℃. Ammonium acetate（0.02 mol/L, pH=5） and acetonitrile were used as mobile phase for gradient elution, an Agilent XDB C18 column was used for separation, and diode array detector was used for the determination of CDG at 449 nm and AOG and AOR at 485 nm with external standard method for quantification. Results Three kinds of basic orange dyes had good linear relationships in the range of 0.01-0.2 mg/L. The average recoveries were 80%-94% and the relative standard deviations were less than 2%（n=6）. The detection limits of the method were 0.1 mg/kg. Conclusion The method is simple and accurate, which is suitable for the determination of basic orange dyes in yuba, dry chili and chili oil.