摘要
从蘑菇培养基质中筛选到一株纤维素酶高产菌株,将其命名为SAISA10,经形态学及分子生物学鉴定,确认该菌为真菌Hypocreales sp.。发酵产酶条件分析结果表明,该菌株最佳发酵条件为:发酵时间3 d、发酵温度40 ℃、pH值4. 0、m(羧甲基纤维素钠):m(麸皮)=1 : 1(g : g)、(NH4)2SO4含量0. 8 g ·(100 mL)^-1 。酶学性质初步研究结果表明,该菌株所产纤维素酶的最适酶解条件为:温度45~55 ℃、pH值4. 0;最佳稳定条件为:温度40 ℃、pH值4. 5。菌株SAISA10是一株高产纤维素酶的真菌,在高温和低pH值下仍有高活性和稳定性,具有较高的开发价值。
A high cellulase-producing strain was screened from the mushroom cultivation matrix,and named as SAISA10. It was identified as Hypocreales sp. by morphological observation and DNA sequence identifica- tion. Results showed that,the optimum fermentation conditions for strain SAISA10 were as follows= fermenta- tion time of 3 d,fermentation temperature of 40 ℃ ,pH value of 4.0,the mass ratio of sodium carboxymethyl cellulose and bran of 1 : l(g :g),(NH4)2SO4 content of 0.8 g · (100 mL)^-1. The preliminary research results of enzymatic properties showed that, the optimum enzymatic hydrolysis conditions were as follows.temperature of 45~55 ℃ ,pH value of 4.0,and the optimum stable conditions were as follows:temperature of 40 ℃,pH val- ue of 4.5. The strain SAISA10 is a high cellulase-producing strain with high activity and stability at high tem- perature or low pH value,which has high exploration potential. Keywords. cellulase ; fungus ; screening ; identification; fermentation condition ; HyDocreales sp.
出处
《化学与生物工程》
CAS
2016年第7期34-39,共6页
Chemistry & Bioengineering
基金
国家自然科学基金青年科学基金资助项目(31401637)
湖南省战略性新兴产业关键共性技术导向类项目(2015GK1017)
湖南农业大学东方科技学院大学生研究性学习和创新性实验计划项目(DFCXY201308)