恶性血液病患者侵袭性真菌感染分子生物学诊断研究

Molecular biological diagnosis of invasive fungal infection in patients with malignant hematologic diseases

目的:了解恶性血液病患者发生侵袭性真菌感染(IFI)的菌种类别,为预防及临床治疗提供科学依据。方法:收集疑似IFI的恶性血液病人痰、血液等标本,分离出真菌40株,采用半巢式基因扩增联合测序法进行鉴定,同时与培养后表型诊断结果进行对比分析。结果:用基因扩增联合测序法40株真菌均鉴别出菌种,鉴定率为100%;用培养后表型法37株鉴定出菌种,3株尚未鉴定出菌种,鉴定率为92.5%。结论:采用基因扩增联合测序法可快速、准确的鉴别出恶性血液患者IFI的菌种类别。

Objective：To understand the species of invasive fungal infection in patients with malignant hematological diseases,to provide scientific evidence for the prevention and treatment.Methods：The 40 strains of fungi isolated from the body fluid samples such as sputum,blood were identified by gene amplification and sequencing and then compared with the diagnosis from culture method.Results：The 40 strains of fungi were identified by gene amplification and sequencing,the rate of identification was 100%;37strains were identified by culture method,3strains couldn＇t be identified,the rate of identification was 92.5%.Conclusion：The species of invasive fungal infection in patients with malignant hematological diseases can be identified quickly and accurately by gene amplification and sequencing method.