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HO-1对SH-SY5Y细胞中α-突触核蛋白和PLK2表达影响 被引量：1

EFFECT OF HO-1 ON THE EXPRESSIONS OFΑ-SYNUCLEIN AND POLO-LIKE KINASE 2 IN SH-SY5Y CELLS
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摘要 目的探讨血红素加氧酶-1(HO-1)对SH-SY5Y细胞中α-突触核蛋白与Polo样激酶2(PLK2)水平的影响。方法采用Western blot方法,检测转染携带HO-1基因片段质粒48h后的SH-SY5Y细胞α-突触核蛋白的表达。给予不同浓度的HO-1激动剂钴原卟啉(CoPPIX)48h后,检测SH-SY5Y细胞PLK2的表达。结果高表达HO-1可降低细胞内α-突触核蛋白水平(t=2.672,P<0.05),10和25μmol/L的CoPPIX通过激活HO-1使PLK2表达上调(F=30.75,q=7.64、10.78,P<0.01)。结论 HO-1可使SH-SY5Y细胞中α-突触核蛋白表达下调,此过程可能与调控PLK2水平有关。 Objective To investigate the effect of heme oxygenase-1（HO-1）on the expressions ofα-synuclein and Pololike kinase 2（PLK2）in SH-SY5 Ycells. Methods Western blot was used to investigateα-synuclein levels in SH-SY5 Ydopaminergic cells with HO-1transfection for 48 hand PLK2levels in cells treated with HO-1activator cobalt protoporphyrin IX（CoPPIX）for 48 h. Results Overexpression of HO-1induced down-regulation ofα-synuclein in SH-SY5Ycells（t=2.672,P〈0.05）.PLK2 levels were significantly up-regulated in cells treated with 10 and 25μmol/L CoPPIX（F=30.75;q=7.64,10.78;P〈0.01）.Conclusion HO-1down-regulates the expression ofα-synuclein,which might be related with the regulation of PLK2 levels.
作者 王冉冉 宋宁 姜宏 谢俊霞
机构地区 青岛大学国家生理学重点(培育)学科
出处 《青岛大学医学院学报》 CAS 2016年第3期294-296,共3页 Acta Academiae Medicinae Qingdao Universitatis
基金 山东省自然科学基金(ZR2014JL022) 青岛市科技成果转化引导计划(14-2-4-61-jch)资助项目
关键词 血红素加氧酶-1 SH-SY5Y细胞 Α突触核蛋白 Polo样激酶2 heme oxygenase-1 SH-SY5Ycells alpha-synuclein polo-like kinase 2
分类号 R742.5 [医药卫生—神经病学与精神病学]
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参考文献12

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青岛大学医学院学报
2016年 第3期

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