子痫前期病人脐血TRPC6和BKCa水平检测及意义

CORD BLOOD TRPC6 AND BKCA IN PATIENTS WITH PREECLAMPSIA:THEIR DETECTION AND SIGNIFICANCE

目的研究子痫前期病人新生儿脐血中瞬时受体电位通道6（TRPC6）和大电导钙激活钾离子通道（BKCa）的水平及其与新生儿出生体质量关系,探讨子痫前期病人胎儿生长受限（FGR）发生与TRPC6、BKCa的相关性。方法采用ELISA方法,检测30例轻度子痫前期、30例重度子痫前期、50例正常妊娠产妇（对照组）新生儿脐血中TRPC6和BKCa水平,并分析其与新生儿体质量关系。结果轻度、重度子痫前期组新生儿脐血TRPC6水平均高于对照组,BKCa水平低于对照组,差异有显著意义（F=6.83、13.27,t=3.05~4.02,P〈0.05）;轻度、重度子痫前期组TRPC6和BKCa水平比较差异无显著性（P〉0.05）。轻度、重度子痫前期组脐血TRPC6水平与新生儿出生体质量呈负相关（r=-0.612、-0.674,P〈0.05）,BKCa水平与新生儿出生体质量呈正相关（r=0.741、0.797,P〈0.05）;对照组脐血TRPC6、BKCa水平与新生儿出生体质量无相关性（P〉0.05）。结论子痫前期病人FGR的发生可能与TRPC6水平升高和BKCa水平降低相关。

Objective To study the levels of transient receptor potential canonical 6（TRPC6）and high conductance calcium-activated potassium channel（BKCa）in cord blood of neonates-their association with birth weigh-and explore the correlation of TRPC6 and BKCa with fetal growth restriction（FGR）in patients with preeclampsia（PCS）. Methods Adopting enzymelinked immunosorbent assay（ELISA）,the levels of TRPC6 and BKCa in neonatal cord blood of 30 patients with mild PCS,30 with severe PCS and 50 normal pregnant women（control group）were detected.The association of TRPC6 and BKCa levels with neonatal body mass was analyzed. Results The cord blood level of TRPC6 in mild and severe PCS was higher than that the control group,and that of BKCa level was lower,the difference was statistically significant（F=6.83,13.27;t=3.05-4.02;P〈0.05）.The difference in the TRPC6 and BKCa level between the mild and severe PCS group was not significant（P〉0.05）.In mild and severe PCS group,cord blood TRPC6 level was negatively correlated with neonatal birth weight（r=-0.612,-0.674;P〈0.05）,and BKCa was positively correlated with neonatal birth weight（r=0.741,0.797;P〈0.05）.In the normal control group,no association of TRPC6 and BKCa with neonatal birth weight was noted（P〉0.05）. Conclusion Fetal growth restriction in patients with preeclampsia may be associated with the increase of cord blood TRPC6 level and the decrease of cord blood BKCa level.