防污剂Irgarol 1051对三角褐指藻生长及生理特性的影响

Effects of Irgarol 1051 on the growth and physiological characteristics of Phaeodactylum tricornutum

为研究防污剂Irgarol 1051对微藻生长及生理生化特性的影响,以三角褐指藻(Phaeodactylum tricornutum)为材料,分析了Irgarol 1051对P.tricornutum生长、叶绿素a含量、可溶性蛋白质含量、超氧化物歧化酶(Superoxide dismutase,SOD)活力及丙二醛(Malondialdehyde,MDA)含量等的影响。结果表明:Irgarol 1051可抑制P.tricornutum的生长,并诱导其产生耐受性;P.tricornutum中叶绿素a、可溶性蛋白质含量及SOD酶活力等均随Irgarol 1051质量浓度的增加而升高,以维持其生长和生理状态;随着Irgarol 1051质量浓度的升高,藻细胞中MDA含量显著增加,表明细胞中活性氧自由基(Reactive oxygen species,ROS)过量积累,这将破坏藻细胞的膜结构与功能。研究结果将为探讨Irgarol1051对微藻的效应机制及其环境风险评价提供数据资料和科学依据。

Effects of different concentrations of Irgarol 1051, an antifouling agent, on changes in the growth and physiological characteristics of Phaeodactylum tricornutum, such as chlorophyll a content, soluble protein content, superoxide dismutase（SOD） activity, and malondialdehyde（MDA） content, were analyzed. Our results showed that Irgarol 1051 significantly inhibited the growth of P. tricornutum and increased its tolerance. Chlorophyll a, soluble protein content, and SOD activity increased with an increase in Irgarol 1051 concentration to protect P. tricornutum cells from Irgarol 1051-induced stress. MDA content in P. tricornutum cells also increased with an increase in Irgarol 1051 concentration, indicating that excessive accumulation of reactive oxygen species damaged the structure and function of the microalgal membrane. Thus, these results provided data to exploit the effects of Irgarol 1051 on microalgae and to evaluate its environmental impact.