桑葚花色苷大孔吸附树脂纯化工艺

Separation and Purification of Mulberry Anthocyanins by Macroporous Resin

通过吸附、洗脱试验,分析大孔吸附树脂对桑葚花色苷的纯化效果,筛选出适合分离纯化桑葚花色苷的大孔吸附树脂,并确立树脂纯化工艺的参数。结果表明,XDA-6型大孔吸附树脂对桑葚花色苷分离效果良好,纯化吸附条件为上清液浓度1.5 mg/mL,pH值3.5,洗脱条件为洗脱剂乙醇溶液浓度60%。

Through adsorption and desorption experiments, purification effects of anthocyanins extracted in mulberry were analyzed to screen for suitable macroporous resin for separation and purification of mulberry anthocyanins, and to establish purification process parameters. Resuhs showed that XDA-6 resin was preferable for separation and purification of mulberry anthocyanins, with the dynamic adsorption conditions was that concentration 1.5mg/mL, original pH3.5, desorption conditions wasthat ethanol concentration 60%.