固定化Cu^(2+)螯合亲和膜的制备及其吸附研究

Preparation and Adsorption Research of Immobilized Cu^(2+)-chelated Affinity Membrane

以尼龙66为基膜,环氧氯丙烷为活化剂,壳聚糖为交联剂,亚胺基二乙酸为螯合剂,Cu^(2+)为配位基,制备了固定化Cu^(2+)螯合亲和膜。讨论了该膜对牛血清蛋白(BSA)的吸附机理;研究了BSA溶液初始浓度、pH值和吸附时间对亲和膜吸附量的影响,采用SEM对其表面形貌进行表征。结果表明:较高的BSA初始浓度和pH值,较长的吸附时间均有利于膜对BSA的吸附;Langmuir吸附等温模型和准二级动力学模型可用于该膜对BSA的吸附相关性。

An immobilized Cu^（2＋）chelated affinity membrane was prepared by using Nylon 66 membrane as substrate membrane, epichlorohydrin as activating agent, chitosan as crosslinker, iminodiacetic acid as coupling agent and copper ion as immobilized metal ion. The adsorption mechanism of BSA was discussed,and then researched the effects of absorption time, initial concentration and p H value of BSA on adsorption of BSA. The surface morphology of immobilized Cu^（2＋）chelated affinity membrane was investigated.The results show that the long adsorption time and high initial concentration and p H value are favorable to adsorb BSA. The Langmuir isotherm and the pseudo-second-order kinetic model can describe the adsorption behavior of BSA better.