胆宁片对非酒精性脂肪肝大鼠的治疗作用

Therapeutic effect of Danning tablets on nonalcoholic fatty liver disease in rats

目的:探讨胆宁片对实验性非酒精性脂肪肝大鼠的治疗作用.方法:SD♂大鼠53只,首先随机分为2组,正常饲料组10只、高脂肪饲料组43只.通过高脂饲料喂养的方式复制非酒精性脂肪肝大鼠模型.4 wk造模结束后,处死10只正常饲料组和3只高脂饲料组大鼠,观察模型复制情况.确定模型复制成功后,将剩余高脂肪饲料组40只随机分为模型组、胆宁片组、绞股蓝组、多烯磷脂酰胆碱组.分别给予相应剂量的生理盐水、胆宁片及各组对应的药物.连续给药4 wk后处死各组大鼠,HE染色法观察病理变化,生化法检测血清甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白(high density lipoprotein,HDL)、低密度脂蛋白(low density lipoprotein,LDL),ELISA法测定肝组织匀浆中肿瘤坏死因子-a(tumornecrosis factor a,TNF-a)、转化生长因子-β1(transforming growth factor-β1,TGF-β1)的含量,分光光度计法测定琥珀酸脱氢酶(succinate dehydrogenase,SDH)的含量.结果:治疗结束后,与模型组比较,治疗各组大鼠肝脏病理学改变有不同程度的改善.与模型组比较,各治疗组大鼠血清中的血脂含量有不同程度的下降(P<0.01),各治疗组肝组织TNF-a、TGF-β1含量下降(P<0.01)、S D H含量升高(P<0.0 1).胆宁片在降低TNF-a、TGF-β1含量、提高SDH方面与多烯磷脂酰胆碱比较无明显差异性(P>0.05),但优于绞股蓝组(P<0.05).结论:胆宁片对非酒精性脂肪肝大鼠有治疗作用.

AIM： To investigate the therapeutic effect of Danning tablets on experimental nonalcoholic fatty liver in rats. METHODS：Fifty-three male rats were randomly divided into two groups,including 10 in a normal diet group and 43 in a high fat diet group. Nonalcoholic fatty liver disease was induced in rats by giving a high fat diet. At the end of 4 wk,10 normal rats and 3 rats in the high fat diet group were killed to determine if the model was established successfully.The remaining rats in the high fat diet group（n = 40） were randomly divided into a model group,a Danning tablets group,a gynostemma group,and a polyene phosphatidylcholine group and given normal saline,Danning tablets and the corresponding drugs,respectively. After 4 wk of treatment,the rats were sacrificed.Pathological changes were examined by HE staining,and biochemical methods were used to detect serum triglyceride（TG）,total cholesterol（TC）,high density lipoprotein（HDL）,and low density lipoprotein（LDL）. ELISA was used to determine the contents of tumor necrosis factor-a（TNF-a） and transforming growth factor-β1（TGF-β1） in liver homogenate,and photometric meter method was used for the determination of the content of succinate dehydrogenase（SDH）.RESULTS： After treatment,compared with the model group,the pathological changes ofthe liver of rats in each group were improved in varying degrees,serum lipid levels showed varying degrees of decrease（P 0.01）,liver tissue TNF-a and TGF-β1 contents significantly decreased（P 0.01）,and SDH increased（P 0.01）. Danning tablets showed similar effects to polyene phosphatidylcholine in reducing TNF-a and TGF-β1 contents and improving SDH（P 0.05）,but was better than gynostemma（P 0.05）.CONCLUSION： Danning tablets have an appreciated therapeutic effect on nonalcoholic fatty liver disease in rats.