不同光周期条件下日本牙鲆尾部神经分泌系统转录组分析

The transcriptome sequencing and functional analysis of CNSS in Paralichthys olivaceus treated with different photoperiods

为发掘日本牙鲆响应光周期变化的重要功能基因,采用新一代高通量测序RNA-seq技术分析8L∶16D、12L∶12D和16L∶8D等3个光周期条件下日本牙鲆尾部神经分泌系统(caudal neurosecretory system,CNSS)的基因表达变化。转录组测序结果显示,3个样品分别产生了5 807 622、6 147 140和6 116 872个Clean reads。分别对8L∶16D、12L∶12D和16L∶8D条件下的文库进行两两比较,共获得200个差异表达基因。GO分类分析表明,差异表达基因属于生物学过程、细胞定位和分子功能的42个类别。KEGG Pathway显著性富集分析差异表达基因共涉及29条代谢途径,包括糖酵解/糖异生、钙离子信号转导、血管平滑肌收缩和光信号转导等通路。上述结果不仅加深了对鱼类尾部神经分泌系统功能的认识,也为进一步探索硬骨鱼类光信号转导机制提供了多方面多层次的信息。

Focusing on discovery and cloning of important functional genes, we summarized a set of bioinformatics methods to efficiently dig for profound RNA-seq information. The results showed that 8L ： 16D, 12L ： 12D and 16L ： 8D had 5 807 622, 6 147 140 and 6 116 872 Clean reads, respectively. Comparison between the sequencing data from 8L ： 16D, 12L ： 12D, and 16L ： 8D revealed 200 differentially expressed genes（DEG）. The DEG GO functions in the transcriptome library were broadly divided into biological process, cellular component and molecular function categories of 42 branches. Data in the transcriptome could be divided into 22 classes taking the KEGG database as a reference, according to the metabolic pathway, including glycocysis/ gluconeogenesis, phototransduction, calcium signaling pathway, and vascular smooth muscle. This study not only conveyed more useful information of CNSS function in flounder, but also provided multi-level information of phototransduction in teleost.