IFNγ释放试验评价水痘减毒活疫苗的细胞免疫效果

Evaluation of cell-mediated immunity of live attenuated varicella vaccine by IFNγ release assay

目的应用IFNγ释放试验评价水痘减毒活疫苗的细胞免疫效果。方法利用IFNγELISA定量试剂盒检测不同灭活抗原(热灭活、紫外灭活、甲醛灭活)刺激、不同孵育时间(24、48、72 h)、不同个体(12人接种水痘减毒活疫苗前后)对IFNγ释放水平的影响。结果紫外灭活方式制备的刺激抗原及新鲜全血与刺激抗原共孵育48 h,IFNγ释放水平最高;12人接种疫苗后全血中IFNγ释放水平均有不同程度提高,接种前后几何平均值(GMT)分别为6.13和234.75 pg/ml,增长倍数和GMT值在个体间均存在较大差异。结论 IFNγ释放试验操作简便、快速,可定量,适用于水痘疫苗细胞免疫效果的评价。

Objective To evaluate the cell-mediated immunity（CMI）of live attenuated varicella vaccine by interferon γ（IFNγ）release assay.Methods The effect of various inactivated antigens（heat-inactivated,ultraviolet-inactivated and formaldehyde-inactivated）,incubation time（24,48 and 72 h）and individuals（12 volunteers before and after immunization with live attenuated varicella vaccine） on the release level of IFNγ were evaluated by quantitative ELISA kit for IFNγ.Results The IFNγ release levels stimulated by ultraviolet-inactivated antigen and by fresh blood co-incubated with ultraviolet-inactivated antigen for 48 h were the highest.The GMTs of IFNγ in blood of 12 volunteers before and after immunization were 6.13 and 234.75 pg/ml respectively.However,the GMTs and their increasing folds showed significant difference in various individuals.Conclusion IFNγ release assay was simple,rapid and quantitative,which was suitable for the evaluation of CMI of varicella vaccine.