骨髓间充质干细胞治疗大鼠白毒伞中毒致急性肝衰竭的效果

Therapeutic effect of bone marrow mesenchymal stem cells on acute liver failure induced by Amanita verna poison in rats

目的 探讨骨髓间充质干细胞（BMSCs）治疗大鼠白毒伞中毒致急性肝衰竭的疗效及机制。方法选取10只体重约80 g的雄性清洁级SD大鼠用于BMSCs的培养。另选取体重在220~250 g的成年大鼠45只作为研究对象,用于肝衰竭模型制备以及BMSCs输注治疗。在45只大鼠中随机抽取15只作为空白对照组,剩下的30只均给予白毒伞粗毒素灌胃制备肝衰竭模型,将造模成功的大鼠随机均分为两组,肝衰竭模型组和治疗组（实验BMSCs输注治疗）。对比各组大鼠7 d生存率及肝功能、凝血功能、炎症因子、肝脏病理等指标情况。结果 与肝衰竭模型组相比,治疗组SD大鼠体重减轻明显更少,7 d生存率明显更高。移植结束后治疗组大鼠肝功能、凝血功能、炎症因子等指标及肝脏病理均较肝衰竭模型组显著改善（P〈0.05）。结论 BMSCs输注对白毒伞中毒致急性肝衰竭大鼠具有十分明显的治疗作用,能显著改善大鼠肝功能衰竭。

Objective To explore the effects and mechanism of bone marrow mesenchymal stem cells （BMSCs） in the treatment of acute liver failure caused by Amanita verna poisoning. Methods Ten clean grade male SD rats （ weight 80 g） were selected as donators of BMSCs. Forty - five adult rats （ weight 220 - 250 g） were selected experimental subjects for hepatic failure model of BMSCs infusion. Fifteen rats were randomly assigned into blank control group, and the other 30 were given with Arnanita toxin gavage for liver failure modeling, as the successful models were further randomly divided into 2 groups, model group and injection treatment group. The 7 - day survival rate, liver function, blood coagula- tion function, inflammatory factors and liver pathology were compared. Results Compared with model group, significantly less weight loss and higher 7 - day survival were revealed in treatment group （ P 〈 0. 05 ）. Significant improvements in liv- er function, blood coagulation, inflammatory factors and liver pathology index were observed in treatment group, when compared with model group （P 〈 0. 05 ）. Conclusion BMSCs are effective in treatment of Amanita poisoning - induced acute hepatic failure in rats, and can significantly improve the liver function.