除湿通痹方对大鼠急性痛风性关节炎抗炎作用的实验研究

Experimental Study on the Anti-inflammatory Effects in Rats with Acute Gouty Arthritis of the Gout One Prescription

目的研究除湿通痹方对尿酸钠(MSU)诱导的急性痛风性关节炎模型大鼠足关节肿胀率及足关节滑膜细胞因子表达的影响,探讨该方治疗急性痛风性关节炎的部分作用机制。方法选用Wistar雄性大鼠40只,随机分成生理盐水组、阳性对照组、除湿通痹方I组、Ⅱ组。各组给予相应药物灌胃,2次/d,第5天灌胃1.5h后予尿酸钠晶体注射局部关节腔形成大鼠急性痛风性关节炎模型,并于不同时间测量大鼠足关节肿胀度,观察该方对大鼠足关节肿胀率的影响;注射尿酸钠24h后,处死大鼠并应用RT-PCR,检测对关节滑膜细胞因子TNF-αm RNA、IL-1βm RNA表达的影响。结果除湿通痹方可有效抑制大鼠踝关节肿胀程度;与生理盐水组比较,TNF-αm RNA和IL-1βm RNA的表达明显减低。结论除湿通痹方可抑制痛风性关节炎TNF-α和IL-1β的表达而发挥抗炎作用,明显减轻关节的病理性损伤,有效抑制关节炎的临床进程。

Objective To investigate the influence of swelling ratio and the expression of cytokines in synovial cell of foot joint of MSU-induced rats with acute gouty arthritis of the gout one prescription, and to investigate the mechanism of the treatment of acute gouty arthritis. Methods Forty Wistar （40） male rates were randomly divided into 4 groups： the normal saline group, positive control group, group I of the gout one prescription and group Ⅱ of the gout one prescription. The corresponding drugs were administered to each group, and 2 times a day, and 1.5 hours after filling the stomach in fifth day, the rats were injected into the Sodium uric acid cavity to form the model of acute gout arthritis in rats, and the swelling degree of the rats was measured at different times to observe the effect of the square on the swelling ratio of the joint; After 24 hours of injection of sodium, rats were executed and the effect of the expression of TNF-a mRNA and IL-1β mRNA in articular synovial cells was detected by RT-PCR. Results The gout one prescription can effectively inhibit the swelling of ankle joint in rats; Compared with the normal saline group, the expression of TNF-a mRNA and IL-1β mRNA was significantly decreased. Conclusion The gout one prescription can inhibit the expression of TNF-a and IL-1β in the gouty arthritis and play an anti-inflammatory effect and it can reduce the pathological damage of the joints and inhibit the clinical course of arthritis effectively.