冰毒成瘾者外周血白细胞端粒长度与中医证素关系的研究

Study on the Relationship between Telomere Length in Peripheral White Blood Cells of Methamphetamine Addicts and TCM Syndrome Elements

目的探讨冰毒成瘾者外周血白细胞端粒长度与中医证素的关系,从而在基因水平上揭示冰毒对人体健康影响的可能中医内在机制。方法研究对象均为年龄在20~49岁的汉族人,试验组为冰毒吸食者136例;对照组为200例健康受试者,规范采集四诊信息,应用证素辨证方法提取中医证素特征和运用QPCR基础技术来测量外周血白细胞的端粒长度。结果 1校正年龄和性别后,冰毒组的端粒长度比健康组的短（P〈0.05）。2冰毒成瘾者的中医证素分布：病位以肝、脾、肾为主,尤以肝最为多见;病性上,实证以气滞最为多见;虚证以气虚最为多见。3校正年龄和性别后,形成肝、湿、热等证素的冰毒组端粒长度与健康组的端粒长度之间均具有明显差异（P〈0.05）,且端粒长度比健康组的端粒长度短。结论 1吸食冰毒会造成端粒损伤,使端粒长度缩短。2冰毒对人体的损伤是多脏腑功能失调共同所致,肝是冰毒成瘾者最主要的病位因素,气滞和气虚是冰毒成瘾者最主要的病性因素。3冰毒成瘾者端粒长度的缩短主要体现在中医肝、湿、热证素的形成上,在基因水平上揭示了冰毒对人体健康影响的可能中医内在机制。

Objective To investigate the relationship between the telomere length in peripheral white blood cells of methamphetamine addicts and TCM syndrome elements, thus exploring effects of methamphetamine on human health of TCM internal mechanism at the gene level. Methods All of the study participants are Han Chinese between the ages of 20 and 49, the experimental group is recruited from 136 cases of methamphetamine addicts and the control group is recruited from 200 cases healthy volunteers. Standard diagnostic information collection and using plain dialectical method of extracting features of TCM syndrome elements, and applying to QPCR based techniques for the measurement of peripheral blood leukocyte telomere length. Results （ 1 ） Correction for age and gender, the telomere length of methamphetamine group is shorter than that in the healthy group（P〈0.05 ）. （2） The distribution of TCM syndrome elements of methamphetamine addicts： disease is mainly distributed in the liver, spleen and kidney, especially in liver most common; the most common case is Qi stagnation of excess syndrome; the most common case is Qideficiency of asthenia syndrome. （3） After adjusting the age and sex, there is significant difference （P〈0.05） among the telomere length of formation of liver, wet and heat syndrome of methamphetamine group and telomere length of adjusted group is more shorter than that in the healthy group. Conclusion （ 1 ） Methamphetamine addicts will cause telomere damage in peripheral white blood cells and will make telomere length short. （2） Methamphetamlne can cause organs dysfunction and disease is mainly in liver; Qi stagnation and Qi deficiency are the most prominent characteristics of disease leading to the methamphetamine addict. （3） The shortened of methamphetamine addicts telomere length is related to the formation of liver, wet and heat syndrome, and reveal the effects of methamphetamine on human health of TCM internal mechanism at the gene level.