投加高效聚磷菌P_2的污泥驯化及其微生物群落变化的研究

Research on the sludge domestication by adding high effective phosphorus- accumulating bacterium P_2 and the change of the sludge microbial community

将高效聚磷菌菌株P2扩大培养后投加到原有污泥当中,通过CASS反应器按照菌株P2的最佳除磷条件进行驯化,并对驯化过程中的相关污水排放指标进行检测,然后对污泥抗冲击能力进行考察,研究其在不同温度、时间、p H及反应参数下的除磷能力,最后对原有污泥及驯化后污泥中微生物的总DNA进行提取,并利用PCR-DGGE变性梯度凝胶电泳技术对其微生物群落结构进行分析。结果表明,当驯化进行到15 d时,出水中磷酸盐含量等各项指标基本趋于稳定,结束第一阶段驯化。驯化系统适宜的p H7.0、适宜的温度为35℃,运行中厌氧好氧交换的频率以20 min为宜,且厌氧释磷时间为1.5 h,此条件下,磷的去除率高达93.6%。在最佳驯化条件下,一些原本在群落中不占优势的聚磷类微生物如聚磷菌P2,经过驯化后能更好适应环境逐渐占据优势地位,活性污泥驯化前后群落结构发生了较大变化。

In order to make the phosphorus removal ability of the activated sludge in the original process further improved,the strain P2 was added to the original sludge after expanding culture,then through CASS reactor according to the approximation optimal phosphorus removal conditions to the strain P2 was domestication,and to detect the related sewage discharge index in the domestication process. When domesticated conducted to 15 d,the various indicators of effluent as phosphorus removal rate were basically stable,ending the first phase of domestication.Then inspect the sludge impact resistance,study on in different temperature,time,p H value and reaction parameters to the phosphorus removal ability,the results showed that the suitable p H was 7.0,a suitable temperature was 35 ℃,the change frequency was 20 min between the anaerobic and aerobic,and the anaerobic time was 1.5 hours.which the phosphorus removal rate reached 93.6%. In the optimum domesticated conditions,some phosphorus accumulating organisms,was not dominant in previous sludge,such as the strain p2,were due to be changed to the main organisms after being domesticated,the activated sludge microbial community structure changed greatly.