动态连续逆流提取鲍鱼腹足胶原蛋白的研究

Dynamic continuous countercurrent extraction of collagen from the foot of Haliotis discus

以次品鲍鱼腹足为原料,采用酸法提取鲍鱼腹足中的胶原蛋白,对其酸溶性胶原蛋白(ASC)的单级动态间歇式提取和多级动态连续逆流提取工艺进行研究与比较。结果表明,单级动态间歇式提取鲍鱼腹足ASC的最佳条件为:1 mol/L醋酸与0.4 mol/L盐酸按1∶1(v/v)配制提取液、料液比1∶10、提取流速40 m L/min、单次提取时间1 h和提取次数2次,在此条件下ASC提取率为11.2%(以干基计),ASC浓度为1.04 mg/m L;而多级动态连续逆流提取的最佳级数为三级,此时ASC提取率可提高至16.9%(以干基计),ASC浓度为3.12 mg/m L。通过调节硫酸铵浓度为0.4 mol/L、p H为5.6对提取所得ASC进行初步纯化,经纯化后ASC纯度最高可达88.4%,回收率为89.1%。

In order to extract acid soluble collagen（ ASC） from the foot of defective Haliotis discus,the process conditions of single- stage dynamic intermittent extraction and multi- stage dynamic continuous countercurrent extraction were investigated and compared. The results showed that the optimum conditions for single- stage dynamic extraction were as follows： acetic acid（ 1 mol / L） / hydrochloric acid（ 0.4 mol / L） 50%（ v / v）,solid to liquid ratio 1 ∶ 10,flow rate 40 m L / min,extraction time 1 h and extraction times 2. Under these conditions,the ASC extraction rate and ASC concentration reached a highest value of 11.2% and 1.04 mg / m L,respectively.Moreover,it was found that three- stages was the best condition for dynamic continuous countercurrent extraction and obtained a higher ASC extraction rate（ 16.9%） and ASC concentration（ 3.12 mg / m L）. Based on these results,ASC was further purified by regulating ammonium sulfate concentration and p H value simultaneously. The highest ASC recovery ratio（ 89.1%） and ASC purity（ 88.4%） were obtained when ammonium sulfate concentration and p H value were controlled at 0.4 mol / L and 5.6,respectively.