硫磺菌凝集素的提取工艺研究

Study on extraction of lectin from Laetiporus sulphureus

硫磺菌凝集素是一种从硫磺菌子实体中提取的、能凝集红细胞的蛋白质。本文以硫磺菌为原料,采用磷酸缓冲溶液浸提,对氯化钠浓度、液料比、提取温度、提取时间、浸提p H五个因素进行单因素实验,并设计四因素三水平的正交实验,以研究硫磺菌凝集素的最佳提取工艺。研究结果表明:氯化钠浓度为0 mol/L,液料比为30∶1 m L/g,提取温度为15℃,提取时间为10 h,浸提p H为7.5的条件下,硫磺菌凝集素的凝集活性最高,达到0.5870,蛋白得率为0.576%。

Lectin from Laetiporus sulphureus was a kind of protein,which could be extracted from Laetiporus sulphureus fruiting bodies and could agglutinate red blood cell. In the paper,the Laetiporus sulphureus was used as a raw material with extraction phosphate buffer. On the basis of single factor experiments,such as sodium chloride concentration,liquid- solid ratio,extraction temperature,extraction time,extraction p H value,four factors and three levels orthogonal experiment was designed to study lectins optimum extraction of the Laetiporus sulphureus. The results showed that the optimum conditions of extraction technology were sodium chloride concentrations of 0 mol / L,liquid- solid ratio of 30 ∶ 1 m L / g,extraction temperature of 15 ℃,the extraction time of10 h,the extraction p H of 7.5.Under the conditions of the extraction,agglutination activity of Laetiporus sulphureus lectin reached 0.5870 and the extraction rate of protein reached 0.576%.