血小板源性生长因子下调肌浆网Ca^(2+)-ATP酶促进人气道平滑肌细胞表型转化

Platelet derived growth factor down-regulated sarcoplasmic reticulum Ca^(2+) atpase to promote the phenotype modulation of human airway smooth muscle cells

目的:探讨肌浆网Ca^(2+)-ATP酶(SERCA2)对人气道平滑肌细胞(cells,HASMCs)表型转化的作用及其机制。方法 :原代培养HASMCs并饥饿5 d后进行分组,分别从显微镜下检测细胞的形态变化、采用Western Blot检测a-actin、SERCA2及ERK通路蛋白的变化、CCK-8检测各组细胞的增殖状态。结果 :与对照组相比,PDGF刺激后可诱导HASMCs收缩蛋白表达减少、增殖能力增加,TSG可显著抑制其作用(P<0.01);PDGF显著抑制SERCA2并促进磷酸化ERK的表达(P<0.01),U0126显著抑制了上述效应(P<0.01)。结论 :PDGF可能通过调节肌浆网Ca^(2+)-ATP酶及ERK通路蛋白的表达诱导HASMCs表型转化。

Objective To investigate of the effect and mechanisman of SERCA2 on the phenotype modulation of HASMCs. Methods HASMCs were starved for 5 days and divided into different groups,then we observed morphology change of the cells from the microscope and detected a-actin,SERCA2 and p-ERK by Western Blot,cells proliferation was observed by CCK-8 method. Results Compared with the control group,PDGF could reduce a-actin of HASMCs and increased the cells proliferation ability,TSG could significantly inhibit the effect （P 〈 0.01）, PDGF could also significantly inhibit SERCA2 protein and increased the expression p-ERK （P 〈 0.01）, while U0126 significantly inhibited the effect （P 〈 0.01 ）. Conclusion PDGF may induce HASMCs phenotype modulation through the regulation of SERCA2 and p-ERK.