不同加工方式的中药白术对脾虚大鼠唾液淀粉酶活性和尿中D-木糖排泄率的影响

Effects of Atractylodis Macrocephale Rhizoma processed by different methods on salivary amylase and D-xylose excretion rate in rats

针对白术的不同加工方法,观察白术在硫磺熏蒸、不同温度鼓风烘制和微波灭菌的加工方式下,样品对脾虚大鼠的唾液淀粉酶及尿中D-木糖排泄率的影响。大鼠分为空白对照组,大黄所致脾虚模型对照组和不同加工方式的白术实验组,分别采用淀粉酶比色测量法和邻苯甲胺法测定大鼠唾液淀粉酶活性和尿D-木糖排泄率。与空白对照组比较,发现100,110℃鼓风烘制的白术均可增加大鼠唾液淀粉酶的单位含量,脾虚大鼠的唾液淀粉酶升高,同时可拮抗大鼠尿中D-木糖排泄率降低的现象,与模型组比较有较明显差异(P<0.05);与模型组相比,70℃烘制及微波处理的白术相对于其他药液作用较强,较模型组有显著性差异(P<0.01)。白术能改善脾虚大鼠对D-木糖吸收功能和唾液淀粉酶活性,且尿中D-木糖排泄率的改变与唾液淀粉酶活性呈正相关。不同温度鼓风烘制和微波灭菌加工的白术对脾虚大鼠唾液淀粉酶活性和尿中D-木糖排泄率的影响较小,而硫磺熏蒸对脾虚大鼠唾液淀粉酶活性和尿中D-木糖排泄率的影响较大。

To observe the effects of Atractylodis Macrocephale Rhizoma processed by different methods （ sulfur-fumigation, different temperatures baking and microwave sterilization） on salivary amylase and D-xylose excretion rate in spleen deficiency rats. The rats were divided into blank control group, rhubarb-induced spleen deficiency model control group, and Atractylodis Macrocephale Rhizoma experimental groups processed with different methods. Amylase colorimetric method was used to determine the activities of salivary am- ylase and D-xylose excretion rate was measured with O-benzylamine method. Then the correlation of salivary amylase activity and D-xy- lose excretion rate in urinary was analyzed. As compared with blank control group, Atractylodis Macrocephale Rhizoma baked at 100, 110 ~C can increase the unit content of rat salivary amylase and D-xylose excretion rate, with a significant difference （P 〈 0. 05 ）. As compared with the model group, Atractylodis Macrocephale Rhizoma baked at 70 ~C and Atractylodis Macrocephale Rhizoma with mi- crowave treatment had stronger effects than the others, with statistically significant differences （P 〈 0. 01 ）. Atractylodis Macrocephale Rhizoma could improve D-xylose absorption function and salivary amylase activity in spleen deficiency rats. In addition, D-xylose ex- cretion rate in urine was positively correlated with salivary amylase activity. Atractylodis Macrocephale Rhizoma processed with different temperatures baking and microwave sterilization had little impact on salivary amylase activity and D-xylose excretion rate in urine of spleen deficiency rats, while sulfur fumigation had great effects on the above two indexes.