不同基原虫草的成分比较及其对人胚胎成纤维细胞抗纤维化作用的初步研究

Study on characteristic spectrum of ingredients from different species Cordyceps and its anti-fibrotic activity on human embryonic fibroblasts

该研究以尿苷、腺苷、虫草素为主要评价指标,采用高效液相、液质联用等色谱方法,对6种不同基原的10个虫草样品的虫体/草体/全体进行高效液相/液质联用的测定和比对;并利用TGF-β1诱导人胚胎成纤维细胞CCC-ESF-1建立体外细胞外基质聚集模型,观察不同基原虫草提取物的抗纤维化活性。实验结果显示,不同基原子实体和菌丝成分数量上基本相同,但是各成分的含量分布具有明显的差异;不同基原的虫草提取物中,体外抗纤维化活性结果为:虫草花>蝉虫草(金蝉花)>桑蚕蛹虫草>柞蚕蛹虫草>天然冬虫夏草。该研究结果为找寻天然冬虫夏草的人工替代品提供了科学的参考和依据。

In this study, 10 samples of parasites, cursive, and the whole from six different species of Cordyceps were determined and compared by HPLC and LC-MS methods. Uridine, adenosine, and cordycepin were selected as the main evaluation index. The an- ti-fibrotic activity of different species Cordyceps extracts was observed using in vitro TGF-β1-induced ECM accumulation in human em- bryonic fibroblasts CCC-ESF-1. The results demonstrated that the number of atoms and hyphae ingredients of different species showed little difference, however, the content distribution of each component has obvious significance. The in vitro anti-fibrotic activities of different species were as follow： Cordyceps flower 〉 Cicada Cordyceps （Cicada flower） 〉 Silkworm Cordyceps 〉 Tussah Cordyceps 〉 natural Cordyceps. Our preliminary data could serve as reference for the discovery of artificial alternatives of natural Cordyceps.