HPLC法测定新疆胀果甘草全草中甘草查尔酮A的含量

Determination of licochalcone A in whole plant of Xinjiang Glycyrrhiza inflate by HPLC

目的对新疆胀果甘草(Glycyrrhiza inflata Bat)全草中甘草查尔酮A(Lico A)进行含量测定。方法用HPLC法对胀果甘草根、茎和叶中Lico A的含量进行测定。色谱柱:依利特Hypersil ODS2C18(250mm×4.6mm,5μm)配预柱;流动相:甲醇-水-冰醋酸(60∶40∶1);流速:1.0mL·min^(-1);柱温:25℃;检测波长:372nm。结果 Lico A质量浓度在6.937 5~111μg·mL^(-1)范围内线性关系良好(r=0.999 9);平均加样回收率99.6%,RSD=1.5%;按此法测得的Lico A含量在根中为0.468%,茎中为0.254%,而在叶中未检出。结论甘草查尔酮A在甘草中主要分布在根和茎中,茎也可作为获取Lico A的新资源。

Objective To measure the content of licochalcone A in the whole plant of Glycyrrhiza inf lata Bat （GIB） from Xinjiang origin .Methods HPLC method was used to perform a quantitative analysis of licochalcone A content in the root ,stem and leaves of GIB plant .Hypersil ODS2 C18 column （250 mm × 4 .6 mm ,5 μm） was used;the mobile phase was composed of methanol‐wa‐ter‐glacial acetic acid （60∶40∶1） mixture ,with a flow rate of 1 .0 mL · min-1 ;the detection wavelength was 372 nm at 25 ℃ . Results Licochalcone A was linear in the concentration range of 6 .937 5‐111 μg · mL -1 （r=0 .999 9）;the average recovery rate was 99 .6% with corresponding RSD of 1 .5% .The content of licochalcone A in the root was 0 .468% ,and in the stem was 0 .254% , however licochalcone A was not detected in the leaf .Conclusion Licochalcone A is mainly distributed in the root and stem of Gly‐cyrrhizainflataBat.Thestemmaybeanew resourceoflicochalconeA.