食用竹炭粉对高脂模型大鼠脂肪代谢的影响

Effects of bamboo charcoal powder on lipid metabolism in hyperlipidaemic rats fed high fat diet

目的探讨食用竹炭粉(bamboo charcoal powder,BCP)对高脂模型大鼠的降脂作用及其机制。方法 4周龄SPF级雄性SD大鼠,随机分成5组:空白对照组,高脂模型对照组和BCP低、中、高剂量(2.81、5.62、11.24 g/kg)干预组。各组每日灌胃相应剂量的竹炭粉或蒸馏水,连续90 d。股动脉取血测定肝肾功能等生化指标;取肝、肾及双侧肾脏及附睾周围的脂肪称重;取左侧肝叶做组织病理学检查;制备肝匀浆,检测总胆固醇(TC)、甘油三酯(TG)、总抗氧化能力(T-AOC)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和丙二醛(MDA)含量。结果从第4周起高脂模型对照组体重显著高于同期空白对照组,同时高脂模型对照组脂/体比、血清谷丙转氨酶(ALT)、TG和极低密度脂蛋白(VLDL)均高于空白对照组;高脂模型对照组肝脏可观察到明显的脂肪变性,肝脏中MDA、TC和TG含量均高于空白对照组,表明造模成功。BCP各剂量组与高脂模型对照组比较,体重、肝重及脂/体比均明显降低,血清ALT、TG和VLDL浓度降低,肝脏脂肪变性程度减轻,同时肝脏中TC和MDA浓度也均降低,T-AOC、SOD和CAT活性升高。结论食用竹炭粉具有减少高脂模型大鼠体内脂肪蓄积、降血脂、减轻肝脏脂肪变性的作用,其机制可能与减轻体内脂质过氧化作用有关。

Objective To evaluate of bamboo charcoal powder（ BCP） on the lipid profile and mechanism in hyperlipidaemia model rats. Methods 40 male Sprague-Dawley（ SD） animals of 4 weeks old were randomly assigned into five groups： the control group fed with low-fat diet; the model control group and the test group（ 2. 81,5. 62,11. 24 g /kg）. Each group gived BCP or distilled water correspondingly,the total administration duration was 90 consecutive days. After the blood samples were collected,liver,kidneys,and white adipose around bilateral epididymis and kidneys were excised and weighed.Serum biomarkers of liver and kidney function were detected. The activities of TC,TGand MDA,T-AOC,CAT,SOD of liver were determined by corresponding test kits according to the manufacturer＇s protocols. Livers were also further detected by macroscopic and microscopic examinations. Results After 90 days of treatment,the weight of rats more than 4 weeks,liver weight and percentage of body fat,serum AST,TG and VLDL,hepatic MDA,TG,TC and liver steatosis in the model control group was all increased compared with the negative control group,indicating that the model has been successfully built. It showed that the weight of rats,liver weight and white adipose weight,serum AST,TG and VLDL,hepatic MDA,TC and liver steatosis in the three dose group was all decreased. The hepatic SOD,CAT,T-AOC in the three dose groups were all increased.Conclusion The BCP could reduce the accumulation of body fat,inmprove blood lipid and hepatic steatosis in hyperlipidemia model rats.