用CGMCC No.8730冠突散囊菌发酵的金花黑茶对两种血脂异常模型大鼠体重和血脂的作用

Effect of CGMCC No. 8730 Eurotium cristatum fermented dark tea extract on body weight and blood lipid in two dyslipidemia rat models

目的研究CGMCC No.8730冠突散囊菌标准化发酵的金花黑茶对大鼠体重和血脂的影响。方法 (1)蛋黄乳剂模型:40只SD雄性大鼠随机分为5组,空白和阴性对照组每日灌胃蒸馏水、其他组每日灌胃低中高(100、500、2500 mg/kg 3个剂量金花黑茶提取物。11 d后禁食并腹腔注射蛋黄乳剂,第12 d采血测血脂,实验期间定期记录体重。(2)高脂饲料模型:50只雄性SD大鼠随机分为5组,空白组饲喂普通饲料,其他组饲喂60 kcal%高脂饲料,空白和阴性对照组每日灌胃蒸馏水、其他组每日灌胃低中高(75、250、750 mg/kg3个剂量金花黑茶提取物。定期记录体重、摄食量和能量摄入,第1、9周末禁食采血测血脂,第9周末取肾周及附睾周脂肪垫,计算体脂比。结果 (1)灌胃12 d后,2500 mg/kg剂量组大鼠的体重和血清甘油三酯水平显著低于蛋黄乳剂模型组(P<0.01)。(2)灌胃9周后,250、750 mg/kg剂量组大鼠的体重和血清甘油三酯水平显著低于高脂饲料模型组,且摄食量、体脂比均降低(P<0.01)。结论 CGMCC No.8730冠突散囊菌标准化发酵的金花黑茶提取物对SD大鼠有控制体重、降低血清甘油三酯的功效。

Objective To investigate the effect of the CGMCC No. 8730 Eurotium cristatum fermented dark tea on body weight and blood lipid. Methods 1. Egg yolk emulsion rat model 40 male Sprague-Dawley（ SD） rats were randomly divided into 5groups,given distilled water or the Eurotium cristatum fermented dark tea extract by intragastric administration for 11 days, then fasted and intraperitoneal injected with distilled water or egg yolk emulsion. The next day rats were taken blood and tested for blood lipid. Body weight were recorded regularly. 2. High-fat diet induced rat model 50 male SD rats were randomly divided into 5 groups fed with normal or 60 kcal% high-fat diet, given distilled water or the Eurotium cristatum fermented dark tea extract byintragastric administration for 9 weeks. Blood lipid was tested at baseline（ 1st week） and endpoint（ 9th week）,and body fat ratio was calculated from perirenal and epididymal fat weight at endpoint. Body weight,food intake and energy intake was weekly recorded.Results 1. Body weight and serum triglycerides of 2500 mg / kg Eurotium cristatum fermented dark tea extract group were significantly decreased foregg yolk emulsion rat model. 2. Body weight,serum triglycerides,food intake and energy intake of 250 mg / kg and 750 mg / kg Eurotium cristatum fermented dark tea extract group were significantly decreased forhigh-fat diet induced rat model. Conclusion The CGMCC No. 8730 Eurotium cristatum fermented dark tea extract decreases body weight andserum triglycerides for SD rats.