半制备型高效液相色谱结合气相色谱-质谱联用法分析卷烟烟气中性香味成分

Analysis of neutral aromaticcomponents from cigarette smoke by semi-preparative high performance liquid chromatography coupled with gas chromatography-mass spectrometry

采用半制备型高效液相色谱结合气相色谱-质谱联用法对卷烟烟气中性香味成分了进行分析。用剑桥滤片捕集卷烟主流烟气粒相物,用同时蒸馏萃取法提取并分离得到中性香味成分。通过对半制备高效液相色谱分离条件和制备馏分体积进行优化,使复杂的烟气中性成分得到预分离,制备出组成相对简单的12个馏分,然后用气相色谱-质谱联用法分析鉴定其中香味成分。结果表明:从各馏分的总离子流色谱图中共鉴定出319种中性成分,其中烃类48种,醛类29种,酮类134种,酯类21种,醇类28种,含氧杂环类18种,含氮化合物32种,酚类7种,醚类2种。方法适合于复杂的烟气中性成分的分析研究。

The neutral aromatic components from cigarette smoke of Golden Leaf brand were analyzed by semipreparative high performance liquid chromatography coupled with gas chromatography-mass spectrometry method.The total particulate matters of cigarette mainstream smoke were collected with Cambridge filter and the neutral aromatic components were extracted by simultaneous distillation and extraction method. In order to separate the complicated neutral components,the separation conditions and fraction volume of semi-preparative HPLC were optimized and 12 fractions with relatively simple composition were prepared. The neutral components in each fraction were analyzed by GC-MS method. The GC-MS analysis results showed that 319 components were identified including 48 hydrocarbons,29 aldehydes,134 ketones,21 esters,28 alchols,18 oxygen heterocyclic compounds,32 nitrogen-containing compounds,7 phenolic compounds and 2 ether compounds. This method is suitable for analysis of complicated neutral components in cigarette smoke.