响应面优化超声波辅助提取刺苋水溶性多糖工艺研究

Optimization of Ultrasonic-assisted Extraction of Water-soluble Polysaccharides from Amaranthus Spinosus by Response Surface Methodology

在单因素试验基础上,利用响应面法考察了超声时间、液料比、超声温度和超声功率四个因素及其之间的交互作用对刺苋水溶性多糖提取率的影响。结果表明超声波辅助提取刺苋水溶性多糖的最佳提取工艺条件为:超声时间35 min,液料比42 m L/g,超声温度73℃,超声功率240 W。在此条件下刺苋水溶性多糖提取率为67.81 mg/g,与预测值(68.23 mg/g)相比,其相对误差为0.616%,说明该方法有效,可靠。

Based on the single -factor experiments, the interactive effects and significance of four variables including ultrasonic time, liquid - to - material, ultrasonic temperature and ultrasonic power were evaluated by response surface methodology analysis. The results showed the optimum conditions were ultrasonic time 35 min, the ratio of liquid - to - material 42 mL/g, ultrasonic temperature 73℃ and ultrasonic power 240W, under these optimized conditions the yield of water - soluble polysaccharides from Amaranthus Spinosus could be up to 67.81 mg/g and the relative error was 0.616% compare to the predicted value （68.23 mg/g）, which proved that the regression equation of the model was effective and reliable.