摘要
Removal of Pb^（2＋）and biodegradation of organophosphorus have been both widely investigated respectively. However, bio-remediation of both Pb^（2＋）and organophosphorus still remains largely unexplored. Bacillus subtilis FZUL-33, which was isolated from the sediment of a lake, possesses the capability for both biomineralization of Pb^（2＋）and biodegradation of acephate. In the present study, both Pb^（2＋）and acephate were simultaneously removed via biodegradation and biomineralization in aqueous solutions.Batch experiments were conducted to study the influence of p H, interaction time and Pb^（2＋）concentration on the process of removal of Pb2＋. At the temperature of 25°C, the maximum removal of Pb^（2＋）by B. subtilis FZUL-33 was 381.31 ± 11.46 mg/g under the conditions of p H 5.5, initial Pb^（2＋）concentration of 1300 mg/L, and contact time of 10 min. Batch experiments were conducted to study the influence of acephate on removal of Pb^（2＋）and the influence of Pb2＋on biodegradation of acephate by B. subtilis FZUL-33. In the mixed system of acephate–Pb2＋, the results show that biodegradation of acephate by B. subtilis FZUL-33 released PO43＋, which promotes mineralization of Pb2＋. The process of biodegradation of acephate was affected slightly when the concentration of Pb2＋was below 100 mg/L. Based on the results, it can be inferred that the B. subtilis FZUL-33 plays a significant role in bio-remediation of organophosphorus-heavy metal compound contamination.
Removal of Pb^（2＋）and biodegradation of organophosphorus have been both widely investigated respectively. However, bio-remediation of both Pb^（2＋）and organophosphorus still remains largely unexplored. Bacillus subtilis FZUL-33, which was isolated from the sediment of a lake, possesses the capability for both biomineralization of Pb^（2＋）and biodegradation of acephate. In the present study, both Pb^（2＋）and acephate were simultaneously removed via biodegradation and biomineralization in aqueous solutions.Batch experiments were conducted to study the influence of p H, interaction time and Pb^（2＋）concentration on the process of removal of Pb2＋. At the temperature of 25°C, the maximum removal of Pb^（2＋）by B. subtilis FZUL-33 was 381.31 ± 11.46 mg/g under the conditions of p H 5.5, initial Pb^（2＋）concentration of 1300 mg/L, and contact time of 10 min. Batch experiments were conducted to study the influence of acephate on removal of Pb^（2＋）and the influence of Pb2＋on biodegradation of acephate by B. subtilis FZUL-33. In the mixed system of acephate–Pb2＋, the results show that biodegradation of acephate by B. subtilis FZUL-33 released PO43＋, which promotes mineralization of Pb2＋. The process of biodegradation of acephate was affected slightly when the concentration of Pb2＋was below 100 mg/L. Based on the results, it can be inferred that the B. subtilis FZUL-33 plays a significant role in bio-remediation of organophosphorus-heavy metal compound contamination.
基金
supported by the National Basic Research Program (973) of China (No. 2014CB846003)
the National Natural Science Foundation of China (Nos. 41372346
21577018)
