泽泻汤对氧化型低密度脂蛋白诱导血管平滑肌细胞增殖的影响

Effect of Alisma Decoction on Vascular Smooth Muscle Cell Proliferation Induced by Oxidized Low Density Lipoprotein

目的观察泽泻汤对血管平滑肌细胞(VSMC)周期蛋白Cyclin D1、Cyclin E、增殖细胞核抗原(PCNA)和p27表达的影响,探讨泽泻汤在氧化型低密度脂蛋白(ox-LDL)诱导的VSMC增殖中的作用和机制。方法通过体外50 mg/L ox-LDL诱导VSMC,建立VSMC增殖的动脉粥样硬化细胞模型;应用空白血清及20%泽泻汤含药血清干预。MTS法检测泽泻汤含药血清对VSMC增殖的影响;Western blot检测增殖相关蛋白Cyclin D1、Cyclin E、PCNA和p27表达水平。结果 50 mg/L ox-LDL可明显诱导VSMC增殖。与ox-LDL组比较,泽泻汤含药血清可显著抑制ox-LDL诱导的VSMC增殖,下调细胞Cyclin D1、Cyclin E和PCNA的表达,同时促进p27蛋白表达。结论泽泻汤具有抗VSMC增殖的作用,机制可能与上调p27蛋白和抑制Cyclin D1、Cyclin E、PCNA表达有关。

Aim To investigate the effect of Alisma Decoction on expressions of cyclin D1,cyclin E,proliferating cell nuclear antigen（ PCNA） and p27 in vascular smooth muscle cell（ VSMC）,and to explore the effect and mechanism of Alisma Decoction in VSMC proliferation induced by oxidized low density lipoprotein（ ox-LDL）. Methods The atherosclerosis cell model of VSMC proliferation was established through induction of VSMC by 50 mg / L ox-LDL in vitro,and was intervened with normal rat serum（ blank serum） and 20% Alisma Decoction-containing serum. The effect of Alisma Decoction-containing serum on VSMC proliferation was detected by MTS method. Expressions of proliferation associated proteins such as cyclin D1,cyclin E,PCNA and p27 were determined by Western blot. Results 50 mg / L ox-LDL could obviously promote VSMC proliferation. Compared with the ox-LDL group,Alisma Decoction-containing serum significantly inhibited VSMC proliferation induced by ox-LDL. Investigation demonstrated that Alisma Decoction-containing serum could decrease the expression level of cyclin D1,cyclin E and PCNA,at the same time,increase the expression level of p27. Conclusion Alisma Decoction can inhibit ox-LDL-induced VSMC proliferation,and its mechanism may be related to increasing p27 expression and inhibiting cyclin D1,cyclin E and PCNA expression.