骨髓间充质干细胞移植对大鼠脑缺血损伤和IL-10、TGF-β1表达的影响

Effect of BMSC Transplantation on the Expression of IL-10 and TGF-β1 in Rats with Cerebral Ischemic Injury

目的观察骨髓间充质干细胞(BMSC)移植治疗缺血性脑卒中大鼠神经功能恢复情况,同时检测血清和脑组织中白细胞介素10(IL-10)和转化生长因子β1(TGF-β1)表达以探讨神经修复的可能机制。方法 36只SD雄性大鼠,随机分为3组:空白组、模型组和实验组。采用改良线栓法制备大脑中动脉闭塞模型(MCAO),实验组缺血24小时后予以BMSC移植干预,模型组给予等量生理盐水,空白组不做处理。分别于移植后第1、3、7、14天不同时间点观察神经行为学评分,通过TTC染色测量脑梗死体积,ELISA及Western blot的方法检测大鼠血清及脑组织中炎症因子IL-10、TGF-β1的变化情况。结果空白组低表达IL-10、TGF-β1;与空白组比较,模型组大鼠神经行为学评分明显升高,脑梗死体积增大(P<0.01),血清及脑组织中IL-10、TGF-β1的表达明显升高(P<0.05);与模型组比较,实验组大鼠脑梗死体积减小(P<0.05),3天、7天及14天中神经行为学评分明显降低(P<0.05),血清及脑组织中IL-10、TGF-β1的表达明显升高(P<0.01),且随着1、3、7、14天的时间的推移,IL-10、TGF-β1表达逐渐下调。结论骨髓间充质干细胞对缺血性脑损伤有修复作用,其机制可能与促进抗炎因子IL-10、TGF-β1的分泌有关。

Aim To observe the protective effects of bone marrow mesenchymal stem cells transplantation for treatment of cerebral ischemia injury in rats,and investigates the expression of interleukin 10（ IL-10） and transforming growth factor beta 1（ TGF-β1） to explore its possible mechanism of nerve repair. Methods 36 male SD rats were randomly divided into blank group,model group,and transplantation group. MCAO model was formed by suture occluded method. Rats intransplatation group were given Bone marrow mesenchymal stem cells. Rats in model group were given the same dose of normal saline and nothing were given to blank group. Evaluate their condition by Bederson scoring at 1,3,7,14 days after injured. Area of cerebral infarction was measured through brain tissue TTC staining. Detect the changes of inflammatory factors IL-10 and TGF-β1 in serum and brain tissue of rats by ELISA and Western blot. Results The blank group had low expression of IL-10 and TGF-β1. Compared with the blank group,both the volume of cerebral infarction of rats in the model group and the scores of the nervous function behavior were increased markedly（ P 0.01）,the expression of IL-10 and TGF-β1 in serum and brain tissue was significantly higher than that in the blank group（ P 0.05）. Compared with the model group,the cerebral infarct volume in the experimental group was decreased,the scores of the nervous function behavior of rats were significantly decreased at 3,7,14 days（ P0.05）,the expression of IL-10 and TGF-β1 in serum and brain tissue was significantly higher than that in the model group（ P 0.01）. With the passage of time,the expression of IL-10 and TGF-β1 was gradually reduced. Conclusions BMSC transplantation is able to improve the recovery of the cerebral ischemic injury. The mechanism may be related to the secretion of anti-inflammatory cytokines IL-10 and TGF-β1.