富血小板血浆对大鼠腹腔巨噬细胞表型的影响及其机制

Effect and mechanism of platelet-rich plasma on phenotype of rat peritoneal macrophage

目的探讨富血小板血浆(PRP)对大鼠腹腔巨噬细胞表型的影响及其可能机制。方法采用含有20%PRP的培养基(实验组)和正常培养基(对照组)体外培养大鼠腹腔巨噬细胞,流式细胞仪检测巨噬细胞表面标记分子CCR7和CD163的表达,ELISA方法检测PRP对上清液中炎症因子IL-4和IL-10表达的影响,Western blot方法检测PRP对巨噬细胞细胞信号传导和转录因子STAT1和STAT6的磷酸化水平的影响。结果与对照组比较,实验组M2型巨噬细胞表面标记分子CD163阳性细胞亚群比例增多,IL-10和IL-4表达增加(P<0.01),磷酸化STAT6蛋白表达上调。结论 PRP能明显促进大鼠腹腔巨噬细胞向M2型转化;其作用机制可能与JAK1/3-STAT6通路被激活有关。

Objective To investigate the effect and underlying mechanism of platelet-rich plasma（PRP） on the phenotype of rat peritoneal macrophage. Methods The rat macrophages were cultrued in 20% PRP（group A） or conventioal medium（group B）. The phenotype of macrophages was evaluated by testing CCR7 and CD163 using flow cytometry. Inflammatory factors of IL-4 and IL-10 were assessd by ELISA. Western blot was used to examine the effect of PRP on p-STAT1 and p-STAT6 expressions. Results Compared with group B, the expression of CD163 was significantly upregulated, the expressions of IL-10 and IL-4 were significantly higher（P〈0. 01）, dhd the expression of p-STAT6 was significantly increased in group A. Conclusion PRP is able to promote the conversion of macrophages to M2 macrophage phenotype. The underlying mechanism may be related with the activation of JAK1/3-STAT6 pathway.