卵泡液中双酚A在多囊卵巢综合征发病中的作用及相关因素探讨

Role of bisphenol A(BPA) in follicular fluid in the pathogenesis of polycystic ovary syndrome(PCOS)

目的:探讨卵泡液中双酚A(bisphenol A,BPA)在多囊卵巢综合征(PCOS)发病中的作用。方法:于取卵日收集行体外受精/卵胞质内单精子显微注射(IVF/ICSI)的PCOS患者和非PCOS患者的卵泡液,检测患者卵泡液BPA水平和卵泡液性激素水平,并进一步与患者血清抗苗勒氏管激素(AMH)及临床结局等因素行相关性分析。结果:PCOS患者和非PCOS患者的卵泡液BPA水平(446.57±63.57 ng/L vs 336.29±59.02 ng/L,P<0.001)、血清AMH水平(12.09±4.78μg/L vs5.69±2.98μg/L,P<0.001)、卵泡液FSH(5.58±1.66 IU/L vs 3.95±1.54 IU/L,P=0.008)、LH(4.54±2.97 IU/L vs 0.96±1.10 IU/L,P<0.001)和T(54.68±34.34 nmol/L vs 32.48±16.68 nmol/L,P=0.035)组间比较有统计学差异。相关性分析显示,卵泡液中BPA水平与血清AMH水平呈显著正相关(r=0.577,P=0.001)。结论:卵巢局部的BPA暴露可能参与了PCOS疾病的发生、发展。

Objective： To explore the role of bisphenol A （BPA） in follicular fluid in the pathogenesis of polycystic ovary syndrome （PCOS）. Methods： BPA concentration was measured in the follicular fluid （FF） collected from non-PCOS and PCOS patients who underwent in vitro fertilization/intracytoplasmic sperm injection （IVF/ICSI）, and the correlation between BPA concentration in FF and serum anti-Mullerian hormone （AMH） level, sex hormone level in FF and clinical outcomes was analyzed. Results： BPA level in FF of PCOS patients was significantly higher than that in non-PCOS patients （446.57 ± 63.57 ng/L vs 336.29± 59.02 ng/L, P〈0.001）. An increase of AMH level in serum was also discovered in PCOS group compared with non-PCOS group （12.09 ± 4.78 μg/L vs 5.69 ± 2.98 μg/L, P〈0.001）. Concentrations of FSH （5.58±1.66 IU/L vs 3.95 ± 1.54 IU/L, P=0.008）, LH （4.54 ± 2.97 IU/L vs 0.96 ±1.10 IU/L, P〈0.001）, T （54.68 ±34.34 nmol/L vs 32.48 ±16.68 nmol/L, P=0.035） in FF of PCOS patients were also higher than those of non-PCOS patients. BPA concentration in FF and AMH level in serum showed a significantly positive correlation （r= 0.577, P=-0.001）. Conclusion： BPA in FF may participate in the pathogenesis of PCOS.