氧化低密度脂蛋白(ox-LDL)、白细胞介素(IL)-22在子痫前期患者血清及胎盘中的表达

Expression of ox-LDL and IL-22 in serum and placenta of patients with preeclampsia

目的:探讨血清及胎盘中氧化低密度脂蛋白(ox-LDL)、白细胞介素(IL)-22的表达与子痫前期(PE)发生的关系。方法:收集PE患者30例,其中轻度PE15例(轻度组),重度PE15例(重度组)。另选同期正常妊娠孕妇30例(正常组),分别采用ELISA、免疫组织化学方法检测血清、胎盘组织中ox-LDL、IL-22的表达水平。结果:1 PE轻度组和重度组患者血清及胎盘中ox-LDL水平均明显高于正常组(P<0.05),且重度组明显高于轻度组(P<0.05);2PE轻度组患者血清及胎盘中IL-22水平较正常组有升高趋势,但差异无统计学意义(P>0.05),而重度组患者血清及胎盘中IL-22水平明显高于正常组及轻度组(P<0.05);3 PE患者血清中ox-LDL与IL-22水平呈明显的正相关(r=0.941,P<0.05);PE患者胎盘中ox-LDL与IL-22水平呈明显的正相关(r=0.754,P<0.05)。结论:血清及胎盘中ox-LDL高表达可能与PE发生、发展有关,而IL-22在此过程中可能起到代偿性保护作用。

Objective： To compared the expression of oxidized low-density lipoprotein （ox-LDL） and interleukin （IL）-22 in serum and placenta between the patients with preeclampsia （PE） and normal pregnant women. Methods： Thirty women with PE were recruited in the study, including 15 women with mild PE （mild group） and 15 women with severe PE （severe group）, and 30 healthy pregnant women were taken as normal group. Enzyme- linked immunosorbent assay （ELISA） was used to measure the serum concentrations of ox-LDL and IL-22 in these women. Immunohistochemical method was used to detect the expression levels of ox-LDL and IL-22 in placenta. Results： 1） The serum concentrations of ox-LDL in mild group and severe group were significantly higher compared with normal group （P〈0.05）; and the concentration of ox-LDL in severe group was significantly higher than that of mild group （P〈0.05）; the same pattem was seen in placenta. 2） The serum concentration of IL-22 in mild group had a trend of increase compared with normal group, but the difference was not statistically significant （P〉0.05）; the serum concentration of IL-22 in severe group was significantly higher than that in normal group and mild group （P〈0.05）; the same pattern was seen in placenta. 3） The serum concentrations of ox-LDL and IL-22 in PE were significantly positively correlated （r = 0.941,P〈0.05）. The expressions ofox-LDL and IL-22 in placenta of PE were significantly positively correlated （r=0.754, P〈0.05）. Conclusion： High expression ofox-LDL in serum and placenta of patients may be related to PE, but IL-22 may be a compensatory protective effect of the body in PE.