还原型谷胱甘肽对高糖诱导的小鼠系膜细胞氧化应激及细胞外基质表达的影响

Effects of Reduced Glutathione on Oxidative Stress and Extracellular Matrix of Mouse Glomerular Mesangial Cells in Hyperglycemic Milieu

目的观察还原型谷胱甘肽（GSH）对高糖诱导的小鼠肾小球系膜细胞氧化应激及细胞外基质表达的影响。方法体外培养小鼠肾小球系膜细胞株分别予正常糖（NG组）、高糖（HG组）、高糖＋GSH（1、5、10mmol／L，高糖＋GSH1—3组）组干预72h；ELISA法测定各组细胞培养上清液中的丙二醛（MDA）、过氧化氢（H2O2）、一氧化氮（NO）、总超氧化物歧化酶（tSOD）和转化生长因子β1（TGF—β1）、纤连蛋白（FN）及层粘连蛋白（LN）的表达情况。结果HG组小鼠肾小球系膜细胞MDA、H2O2、NO、TGF—β1、FN、LN的表达水平均较NG组增加，tSOD表达降低（P〈0．05）；高糖＋GSH1～3组MDA、H202、FN、LN表达较HG组减少（P〈0．05），高糖＋GSH2、3组NO、TGF—β1表达较HG组降低（P〈0．05），高糖＋GSH3组tSOD表达较HG组增加（P〈0．05）。结论GSH可改善高糖诱导的系膜细胞氧化应激反应及减少细胞外基质积聚，对糖尿病肾病可能有一定防治作用。

Objective To observe the effects of reduced glutathione （GSH） on oxidative stress and extracellular matrix of mouse glomerular mesangial cells in hyperglycemic milieu. Methods The cultured mouse glomerular mesangial cells were divided into 5 groups： normal glucose group （5mmol/L） , high glucose group （30mmol/L） , and GSH groups （high glucose with 1 mmol/L, 5mmol/L, 10mmol/ L GSH respectively）. The cell culture supernatant were saved for detecting the concentrations of MDA, H202 , NO, tSOD and TGF -β1, FN, LN at 72h after different treatments by ELISA assay. Results Compared with the normal glucose group, the concentrations of MDA, H202, NO and TGF-β1, FN, LN were increased and the concentration of tSOD was decreased in high glucose group （P 〈0.05）. The concentrations of MDA, H2O2 , FN and LN were reduced by treatment of different concentrations of GSH （P 〈 0.05） , and the concentra- tions of NO, TGF - β1 were inhibited by GSH 5 and 10mmol/L, and the concentration of tSOD was increased by 10mmol/L of GSH （P 〈 0. 05）. Conclusion GSH could inhibit oxidative stress and extracellular matrix of mouse glomerular mesangial cells induced by hyperglycemic milieu, and may be helpful in the treatment of diabetic nephropathy.