摘要
以玉米黄粉为原料,利用Alcalase酶、Protamex酶和Flavourzyme酶酶解制备血管紧张素转换酶(ACE)抑制肽,研究酶解条件、酶解方式对玉米肽ACE抑制活性的影响。同时,在试验的基础上,对100kg玉米黄粉酶解制备ACE抑制肽进行了中试生产。实验结果表明:单酶水解制备ACE抑制肽的最适酶为Protamex酶,在酶解150rain时水解产物的ACE抑制率为(90.98±0.54)%,水解产物的分子质量集中在10405.22—300Da。Flavourzyme酶和Protamex酶的分步水解可进一步提高玉米蛋白的酶解效率和玉米肽对ACE的抑制活性,在先用Flavourzyme酶水解2h再加入Protamex酶继续水解3h后,水解产物的ACE抑制率达到(99.35±3.55)%(IC50=0.764mg/mL),水解产物的分子质量集中在8439.49~185.2Da。采用双酶分步水解方式水解100kg玉米黄粉时,肽粉得率为27%,ACE抑制率为(56.70±0.86)%(1mg/mL)。
In the present study, corn gluten meal (CGM) was hydrolyzed using three proteases (Alcalase,Protamex and Flavourzyme) to produce the ACE-inhibitory peptide. Effect of hydrolysis conditions and methods on ACE- inhibitory activities of corn peptide was investigated. Meanwhile, on the base of small scale experiment, ACE-inhibitory peptide from hydrolyzing 100 kg CGM was prepared. The results showed that Protamex protease was the optimal enzyme for the production of ACE-inhibitory peptide when using one-step hydrolysis, and under the condition of 150 min of hydrolysis, the inhibition rate of ACE was 90.98% ±0.54% , the molecular weight of peptides in the hydroly- sate was mainly distributed from 300 Da to 10 405.22 Da. The hydrolysis efficiency of corn protein and ACE-inhibitory activity of corn peptides were increased by two-step enzymatic hydrolysis of Flavourzyme and Protamex proteases. 1The first-step enzymatic hydrolysis with Flavourzyme proteases was carried out for 2 h, the mixture was subjected to a second-step enzymatic hydrolysis with Protamex proteases for another 3 h, the ACE inhibition activity of hydrolysate reached to 99.35% ± 3.55% (ICs0 =0. 764 mg/mL) , the molecular weight of peptides was mainly distributed from 185.2 Da to 8 439.49 Da. In pilot-scale production, two-step enzymatic hydrolysis was applied to prepare ACE-inhibitory peptide, yield of peptide power was 27% and the inhibition rate of ACE was 56.70% ±0.86% at 1 mg/mL.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2016年第7期158-164,共7页
Food and Fermentation Industries
基金
齐齐哈尔市科学技术计划项目(GYGG-201306)
黑龙江省自然科学基金(C201332)
关键词
玉米黄粉
ACE抑制率
分步水解
分子质量分布
中试生产
corn gluten meal
inhibitory rate of ACE
two-step enzymatic hydrolysis
molecular weight distribution
pilot-scale production