视网膜干细胞移植对青光眼视网膜神经节细胞的保护

Protective effect of retinal stem cell transplantation on retinal ganglion cells in glaucoma

背景:干细胞移植是治疗致盲性眼病的一种新方法,关于视网膜干细胞移植对青光眼视网膜神经节细胞保护作用的研究不多。目的:探讨视网膜干细胞移植对青光眼大鼠视网膜神经节细胞的保护作用。方法:将45只SD大鼠随机分为3组(n=15):对照组、模型组、视网膜干细胞移植组,后2组建立青光眼大鼠模型,视网膜干细胞移植组大鼠在建模后第7天向玻璃体腔内注射1 mL视网膜干细胞(5×106个细胞),模型组大鼠注射等量的PBS,对照组大鼠不予任何处理,移植2周后进行相关指标检测。结果与结论:(1)模型组脑源性神经营养因子和胰岛素样生长因子Ⅰ蛋白的表达均明显低于对照组(P<0.05),视网膜干细胞移植组脑源性神经营养因子和胰岛素样生长因子Ⅰ蛋白的表达明显高于模型组(P<0.05),但仍低于对照组(P<0.05);(2)模型组的视网膜神经节细胞的凋亡数明显高于对照组(P<0.05),视网膜干细胞移植组的视网膜神经节细胞的凋亡数明显低于模型组(P<0.05),但仍高于对照组(P<0.05);(3)模型组的视网膜神经节细胞数明显低于对照组(P<0.05),视网膜干细胞移植组的视网膜神经节细胞数明显高于模型组(P<0.05),但仍低于对照组(P<0.05);(4)结果表明,视网膜干细胞移植治疗大鼠青光眼可以保护视网膜神经节细胞。

BACKGROUND：Stem cel transplantation is a new method for blinding eye disease. But there is a lack of research about the protective effect of retinal stem cel transplantation on retinal ganglion cel s in glaucoma. OBJECTIVE：To explore the protective effect of retinal stem cel transplantation on retinal ganglion cel s of rats with glaucoma. METHODS：Forty-five Sprague-Dawley rats were randomly divided into three groups （n=15 per group） including control, model and retinal stem cel transplantation groups. Rat models of glaucoma were prepared in the latter two groups, and at 7 days after modeling, rats in the three groups were given intravitreal injection of 1 mL retinal stem cel s （5x106 cel s）, the same amount of PBS, and no treatment, respectively. Subsequently, relative indicators were detected at 2 weeks after transplantation. RESULTS AND CONCLUSION：The expressions of brain-derived neurotrophic factor and insulin-like growth factor I protein as wel as the number of retinal ganglion cel s were the highest in the control group, fol owed by the retinal stem cel transplantation group model group, and the lowest in the model group （P〈0.05）. The number of apoptotic retinal ganglion cel s in model group was significantly higher than that of control group （P〈0.05）, and which in the retinal stem cel transplantation group was significantly lower than that in the model group （P〈0.05）, but higher than that in the control group （P〈0.05）. These results suggest that retinal stem cel transplantation for rat glaucoma can exert a protective effect on retinal ganglion cel s.