饲料添加剂的蓄积对幼犬睾丸功能相关酶活性的影响

The Influence of Accumulation of Feed Additive on Puppies Testicular Function Marker Enzyme Activity

探讨饲料添加剂的蓄积对雄性幼犬的生长、生殖发育的影响。选用1月龄土犬12只,随机分为试验组和对照组,试验组幼犬饲喂正常剂量日粮和高剂量组大鼠鼠肉,对照组幼犬饲喂正常剂量日粮和大鼠鼠肉,喂至幼犬成熟,检测雄性幼犬食用饲料添加剂高剂量组大鼠鼠肉后对其生长、生殖发育产生影响。幼犬睾丸标志酶活性结果表明,试验组幼犬睾丸组织中酸性磷酸酶(ACP)和乳酸脱氢酶(LDH)的酶活性下降,碱性磷酸酶(AKP)活性未发生变化;试验组的ACP和LDH活性下降,差异极显著(P<0.01)。

This experiment aimed to study and analysis the feed additives widely used on the effect of feedadditives accumulation on growth and reproductive development of the second- feed male pups. Tweleve one-month-old male pups were selected and randomly divided into experimental and control groups. The control groupwere fed with the normal diet and rat meat, the experimental group were fed with the normal diet and high-dose ratmeat. After the dogs mature, and tested high-dose rat meat effect on male pups growth and reproductive development.Detected testicular function marker enzyme activity and the results showed that the experimental group testiculartissue lactate dehydrogenase, acid phosphatase activities were having a downward trend, the experimental group ofalkaline phosphatase viability did not change. Compared with the control group, LDH viability experimental groupwas significantly lower, the difference was statistically significant; ACP vitality experimental group was significantlylower, the difference was statistically significant（P0.05）.