高分辨率显微内镜对动物胃肠道黏膜成像的可行性研究

Feasibility of high-resolution microendoscopy imaging for animal gastrointestinal mucosa

目的探索高分辨率显微内镜（HRME）对动物胃肠道黏膜成像的可行性。方法选择日本大耳白兔胃、小肠、大肠组织黏膜为研究对象，进行HRME成像，观察胃窦、胃底、小肠和大肠黏膜HRME的成像效果。调整曝光时间，比较不同曝光时间对HRME成像效果的影响。成像结果与病理结果进行对比。结果动物胃肠道黏膜经HRME成像后，通过对图片进行分析可以清晰的区分不同组织。胃底黏膜：浅层可见大量排列紧密的腺体，胃小凹开口呈类椭圆形或长形分枝状，周边裂隙呈线样，细胞核排列规则。胃窦黏膜：浅层可见胃小凹开口呈不规则形或管状，腺腔呈裂隙状，小凹周围细胞排列规则，细胞核小且分布密集。小肠黏膜：浅层可见绒毛呈宽大的指状，立体感明显，呈簇状排列，间隙呈裂隙状，表面覆盖排列规则的发亮的吸收细胞。大肠黏膜：浅层可见排列规则的大量菊花样圆形隐窝，大小基本一致，隐窝周边隐约可见发亮的杯状细胞，隐窝间隙清晰可见。细胞核显像随着曝光时间的延长而变得更加明亮。病理检查结果与HRME结果具有良好的一致性。结论通过HRME对动物胃肠道黏膜进行成像取得了较好的成像效果，为进一步用于人体组织成像研究提供了理论依据。

Objective To investigate the feasibility of high-resolution microendoscopy（HRME） im- aging for animal gastrointestinal mucosa. Methods Mucosal tissues were harvested from the stomach, small intestine, and large intestine of Japanese big-ear white rabbits. The effects of HRME imaging of different lo- cations such as the gastric antrum and fundus, small intestine and large intestine were observed, and those of different exposure time were compared.Accuracy of HRME imaging was compared with pathology. Results The specific tissues of the gastrointestinal mucosa could be clearly distinguished from the HRME images. In the superficial layer of the fundic mucosa, numerous closely arranged glands as well as oval or elongated branched openings of the gastric pits and linear peripheral cracks were visible; the nuclei were arranged reg- ularly. In the superficial layer of the antral mucosa, irregular or tubular openings of the gastric pits and cracked glandular cavities were visible, with the ceils surrounding the gastric pits regularly arranged and the nuclei small and densely distributed. In the superficial layer of the small intestine mucosa, stereoscopic thick-finger-shaped villi cluster was visible. The intervillous spaces were crack-like, and the surface was cov- ered by regularly arranged reflective, absorptive cells. In the superficial layer of the large intestine mucosa, many regularly arranged daisy-like round crypts of uniform size, as well as reflective, goblet ceils surrounding the crypt and the interval space between crypts were visible. When the exposure time increased, the nuclei became brighter. An excellent correlation was noted between the results of histologic examinationand those obtained by using HRME. Conclusion HRME can produce accurate images of the animal gastro- intestinal mucosae and may be a novel technique for further studies of human gastrointestinal pathology.