河北省卢龙县2010—2014年婴幼儿病毒性腹泻病原学分析

Pathogen detection for viral diarrhea among infants in Lulong county of Hebei province,2010-2014

目的 研究河北省卢龙县婴幼儿病毒性腹泻病原构成。方法 2010—2014年对卢龙县县医院所有〈5岁住院腹泻儿童进行全年监测,对病毒性腹泻患儿收集流行病学信息和腹泻粪便采样进行病原学分析。结果共收集1 880份腹泻样本,4种病毒阳性样本1 356份,阳性率平均为72.1%,其中轮状病毒（HRV）占45.0%（846份）,杯状病毒（HuCV）占18.3%（344份）,腺病毒（Ade V）占6.3%（119份）,星状病毒（AstV）占2.5%（47份）。1月—翌年12月为HRV发病高峰,1—3月为HuCV发病高峰,11月—翌年4月为Ade V发病高峰,2—3月为Astv发病高峰。HRV、HuCV、Astv均以12~月龄组病例最多,分别占38.1%、32.6%、38.2%,而Ade V的患儿以9~月龄组病例最多,占21.8%。轮状病毒G9血清型为流行毒株（57.1%）,基因型P[8]为主（83.7%）。HuCV以GⅡ组为优势株。结论 HRV是婴幼儿腹泻主要病原。

Objective To study pathogens of viral diarrhea among infants in Lulong county of Hebei prov- ince. Methods Medical records of all diarrhea patients aged less than 5 years and hospitalized in Lulong County Hospi- tal during 2010 and 2014 were collected and stool specimens of the inpatients were collected for viral pathogen detection with enzyme-linked immunosorbent assay （ELISA） and revere-transcription polymerase chain reaction （RT-PCR）. Re- suits For the 1 889 stool specimens detected, the overall virus positive rate was 72. 1% （ 1 356） and the positive rate of human rotavirus （ HRV ）, human caliciviruses （ HuCV ）, adenovirus （ AdeV ）, and astrovirus （AstV） were 45.0% （ 846 ）, 18.3 % （ 344）, 6. 3 % （ 119 ）, and 2. 5 % （ 47 ）, respectively. More positive specimens were detected during December to January of the next year for HRV, January to March in a year for HuCV,November to April of the next year for AdeV, and February to March in a year for AstV. More than or near one third of the HRV, HuCV, and AstV positive cases were aged 12 - 17 months, with the proportion of 38.1%, 32. 6%, and 38.2% ; while more than one fifth （21.8%） of AdeV positive cases were aged 9 - 11 months. The dominant serotype of HRV strains identified was G9 （57.1% ）, with a dom- inant genotype of P [ 8 ] ; the dominant serotype of the HuCV strains was G I1. Conclusion HRV is a main pathogen of diarrhea among infants in Lulong county of Hebei province from 2010 to 2014.