Twist和ZO-1在卵巢癌中的表达及其与卵巢癌发生发展的相关性研究

目的研究Tlwist和z0．1在卵巢癌和正常卵巢组织中的表达及其分布特点，并探讨二者在卵巢癌表达中的相关性及其与肿瘤发生发展的关系。方法采用免疫组织化学方法（SP法）检测卵巢癌患者手术切除标本癌组织及正常卵巢组织中Tlwist和ZO-1的表达水平，并与肿瘤的分级和分期进行统计学分析。结果Twist在卵巢癌组织中阳性表达率（76．0％，38／50）高于正常卵巢组织（20．O％，4／20），差异有统计学意义（P〈O．05）；ZO-1在卵巢癌组织中阳性表达率（32％，16／50）低于正常组织（85．0％，17／20），差异有统计学意义（P〈O．05）；Twist和ZO·1的表达水平在卵巢癌的不同分级和分期中的表达差异有统计学意义（P〈O．05）；二者在卵巢癌中的表达呈负相关（r=-0．76，P〈O．05）。结论Twist和ZO-1在卵巢癌组织中表达的变化可能与肿瘤的发生发展有关；Twist和ZO-1联合检测可作为评估卵巢癌转移与预后的指标。

Objective To investigate the expression and distribution ＆Twist and ZO-1 in epithelial ovarian cancer and to explore the correlation hetweenTwistandZO-1 in ovarian cancer. Methods lmmunohistoehemicalmethod （SPmethod） was used to detect the expression ofTwist and ZO-1 in 50 cases of ovarian cancer tissue and 20 cases of normal tissue of patient.The clinicopathologic characteristics and tumor staging were statistically analyzed. Results The positive rate of Twist was76.0% （ 38/50 ） in ovarian cancer, 20.0 % （ 4/20 ） in the normal tissue of other patients, There were significant differences （ P〈0.05 ） .The positive rate of ZO-1 was 32.0% （ 16/50 ） in ovarian cancer, significantly lower than that in the normal tissue 85.0% （ 17/20 ） （ P〈0.05 ） .The expression of Twist and ZO-1 were associated with tumor staging and grading in ovarian cancer patients （ P〈0.05 ） . There was a negative correlation between the expression of Twist and ZO-1 in ovarian cancer patients. Conclusions The the high express of Twist and low express of ZO-I may be concerned with the genesis and the development of human epithelial ovarian cancer.The combined detection of Twist and ZO-1 expression may be used in assessing the metasasis and predicting the clinical prognosis of adenocarcinoma ovarian cancer.