盐酸米诺环素软膏对微螺钉支抗种植体周围炎龈下伴放线放线杆菌影响的荧光实时定量PCR检测

目的探讨盐酸米诺环素软膏对微螺钉支抗种植体周围炎龈下伴放线放线杆菌（Aa）影响。方法选取2013年1月至2015年1月15例微螺钉支抗治疗后种植体周围炎的患者，共20种植体，设为观察组；选取同期接受微螺钉支抗治疗的患者14例，共20种植体，经证实无种植体周围炎，设为对照组。记录两组菌斑指数（PLI）、探诊深度（PD）及龈沟出血指数（SBI）。采用实时荧光定量PCR检测Aa。结果观察组PLI、PD和SBI均显著高于对照组（P〈0．01）；治疗1、3N后观察组PLI、PD和SBI显著降低，治疗6周后有上升趋势，但与治疗前相比差异有统计学意义（P〈0．05）。治疗前，观察组Aa16srDNA数量显著高于对照组（P〈0．01）；治疗1、3周后观察组Aa16srDNA数量显著降低，与治疗前相比差异有统计学意义（P〈0．05），治疗6N后有上升趋势，与治疗前相比差异无统计学意义（P〉0．05）。结论盐酸米诺环素软膏治疗微螺钉支抗种植体周围炎，可明显改善种植体周围炎临床症状，疗效确切，其对Aa具有较强的抗菌效果。实时定量PCR是检测龈下菌斑中Aa的可靠方法。

Objective To investigate the effect of minocyeline hydrochloride ointment on Actinobacillus actinomycetemeomitans for patients with mieroserew implants peri-implantitis. Method 15 cases of patients （ with 20 microscrew ） with microscrew implants peri-implantitis from Jan 2013 to Jan 2015 were used as observation group and another 14 eases （ with 20 microserew ） without microserew implants peri-implantitis were used as control group. Plaque index （ PLI ） , probing depth index （ PD ） and suleus bleeding index （ SBI ） of two groups were determined.Real-Time PCR was used to inspect Actinobaeillus actinomycetemeomitans.Results Before and after treatment, the PLI, PD and SBI of observation group were significantly higher than that of control group （ P〈0.01 ） . After treatment for 1st and 3rd weeks, the PLI, PD and SBI of observation group significantly decreased and increased at 6th weeks without significant difference （P〈0.05） .There was significant difference between the 16s rDNA of Aetinobacillus actinomycetemeomitans before treatment （P〈0.01） .After treatment, the 16s rDNA of Actinobaeillus aetinomycetemeomitans of observation group significantly decreased （ P〈0.05 ） .Conclusion Treatment on microscrew implants peri-implantitis with minocycline hydrochloride ointment could improve clinical symptom and inhibitory effect on actinobaciUus actinomyeetemeomitans.