采用炭黑水热法制备荧光碳点

Hydrothermal Synthesis of Fluorescent Carbon Dots From Carbon Black

主要采用水热法来合成高荧光和高量子产率（QY=25.44%）的水溶性碳点。以炭黑作为主要原料,以乙二醇作为钝化试剂和修饰试剂,在120℃温度下加热制备碳点。此方法合成的碳点具有激发依赖性,即发射波长会随着激发波长的红移而不断红移,从而发出蓝色,绿色,黄色,红色等不同颜色的荧光。而且合成的碳点的粒径较小,只有2~3nm,方便进入细胞。利用洋葱表皮细胞对合成的碳点进行细胞成像试验,结果表明碳点可以顺利地穿过细胞壁、细胞膜、核膜等三重障碍进入细胞核,并对细胞核进行特异性标记,有利于在生物医学领域的应用。

Facile hydrothermal synthesis of water soluble carbon dots with high fluorescence and high quantum yield（QY=25.44%）using commercial carbon black as a precursor and glycol as a passivating agent at 120 ℃ was reported.The fl uorescence changed from blue,green,yellow,orange and red with excitation wavelength shifting.Carbon dots with a particle size of 2~3nm are easy to get into cells.Onion epidermal cells were used to evaluate bioimaging and labeling capabilities of carbon dots here.The results showed carbon dots synthesized in this way can enter cells,and even cell nucleus through cell walls,cell membranes and nuclear membranes.The confocal fluorescence microphotographs demonstrate that the carbon dots can be used to label cell nucleus specifi cally and favour application in biomedical fi eld.