构建含5-氟尿嘧啶的自组装短肽原位水凝胶的初步流变学研究

Rheology study on construction of in-situ hydrogel containing 5-fluorouracil with self-assembling peptide

目的探讨构建含5-氟尿嘧啶(5-Fu)的自组装短肽RAD16-I原位水凝胶的可能性。方法采用流变仪测量短肽水溶液与磷酸盐缓冲液(PBS)混合前后的储存模量、损耗模量、相位角等流变学参数,表征含5-Fu的RAD16-I水溶液在模拟生理条件下形成水凝胶的情况;通过倒置显微镜观察含5-Fu的RAD16-I水溶液加入细胞培养基后形成的水凝胶及细胞的形态,考察含5-Fu的RAD16-I溶液在细胞培养基中形成水凝胶、维持凝胶状态的情形和水凝胶中药物对细胞的作用。结果不含和含5-Fu的RAD16-I水溶液在PBS或细胞培养基中均能形成凝胶,凝胶呈透明的膜片状,在培养基中与细胞共处时,含药水凝胶较好地维持其凝胶状态和5-Fu的抗肿瘤效果。结论自组装短肽可以作为5-Fu等抗肿瘤药物的载体材料加以开发。

Objective To investigate potential of self-assembling peptide RAD16-I as in-situ hydrogel for 5-fluorouracil.Methods Hydrogel formation of 5-fluorouracil-contained RAD16-I solution and the effect of 5-fluorouracil in the hydrogel were studied through rheology study on hydrogel and microscopic observation on hydrogels and cells cultured in the hydrogels.Results Hydrogel with certain mechanical intensity formed rapidly in 5-fluorouracil contained RAD16-I solutions immediately after they were mixed with PBS.Also,in cell culture media,RAD16-I solutions containing 5-fluorouracil can form hydrogels,which can keep their gel form and effects on cancer cells in the cell culture media.Conclusion The self-assembling peptide may be explored as in-situ hydrogel carrier for anti-cancer drugs.