细胞因子IL-27和CpG ODN佐剂对呼吸道合胞病毒重组蛋白疫苗增强性肺部免疫病理的调节作用

Regulatory effects of IL-27 and Cp G ODN adjuvant on RSV recombinant vaccine-enhanced lung immune pathology

目的研究细胞因子佐剂IL-27单独或与Cp G2216佐剂联合使用对呼吸道合胞病毒(RSV)重组蛋白疫苗G1F/M2免疫病理的调节作用。方法将编码IL-27的质粒(以下简称IL-27)单独或与Cp G2216佐剂联合使用与G1F/M2共免疫Balb/c小鼠3次,第3次免疫后第10天用RSV攻击免疫后的Balb/c小鼠,攻毒后5 d处死,留取肺组织,用实时定量PCR检测RSV-N基因的表达量,Th1和Th2型细胞因子IFN-γ和IL-5,中性粒细胞和嗜酸性粒细胞趋化因子Gro-α和eotaxin,Th1和Th2特异性转录因子T-bet和GATA3及黏液因子gob-5的m RNA的表达量;另一部分肺组织做病理切片,用HE染色观察肺组织情况。结果 PBS组明显被RSV感染,而各疫苗组显著被保护。IL-27+G1F/M2组和IL-27+Cp G2216+G1F/M2组的IFN-γ表达量无差异(P>0.05),且均显著高于无佐剂的G1F/M2组(P<0.05);IL-27+Cp G2216+G1F/M2组的T-bet表达量显著高于其它组(P<0.05);IL-27+Cp G2216+G1F/M2和IL-27+G1F/M2组的GATA3、IL-5表达量均显著低于G1F/M2组(P<0.05),且IL-27+G1F/M2组的IL-5表达量在疫苗组中最低(P<0.05);IL-27+G1F/M2组的Gro-α、eotaxin、gob-5表达量显著低于其它疫苗组(P<0.05)。病理切片结果显示:与正常小鼠相比,各组经RSV攻击后肺组织均有不同程度的炎症病理损伤,而IL-27+G1F/M2组的肺部的炎症病理损伤与其它各组相比明显减轻。结论 IL-27+Cp G对疫苗增强性肺部免疫病理有一定的下调作用,而IL-27能显著抑制疫苗增强性肺部免疫病理。

This study was designed to investigate the regulatory effects of IL-27 or/and CpG ODN adjuvants on the protective efficacy and safety of RSV recombinant protein vaccine. Balb/c mice were immunized with RSV vaccines G1F/M2 plus pcDNA3.1/IL-27 alone or in combination with CpG ODN three times at an interval of 10 days, and was phlebotomized at day 10 post-immunization. Ten days after the third immunization, mice were challenged with RSV, and sanctified 5 days later. Samples of lung were collected, and RNA was extracted. RSV-N gene, cytokines INF-γ, IL-5, Gro-α, eotaxin, T-bet, GATA3 and gob-5 were tested by fluorescence base real-time PCR; while ections of paraffin embedding lung tissues were stained by HE for histological analysis. Data showed that mice in every group were protected against RSV challenge except mice in PBS group, and the protective efficacy of G1F/M2 with adjuvants was better than that of G1F/M2 without adjuvant. The expression of IFN-γ/in IL-27＋G1F/M2 group and IL-27＋CpG2216＋G1F/M2 group were higher than that in G1F/M2 group, and there was no difference between the two adjuvat groups. IL-27 ＋ CPG2216 ＋ G1F/M2 induced higher levels of T-bet than other groups do （P〈0.05）; the mRNA levels of GATA3 and IL-5 in IL-27＋CpG2216＋G1F/M2 group and IL-27 ＋G1F/M2 group were significantly lower than those in G1F/M2 gruop （P〈0.05）; the mRNA level of IL-5 in IL-27 ＋GIF/M2 group was dramatically reduced as compared with vaccine groups; the mRNA levels of Gro-α; eotaxin and gob-5 in IL-27＋G1F/M2 group was lower than those of other vaccine groups （P〈0.05）. Histopathology result showed that except normal control group, lung tissues in the other groups showed pulmonary inflammatory following RSV infection, while those in IL-27＋G1F/M2 group was the fewest. In conclusion, IL-27 plus CPG2216 can reduce vaccine enhanced immunopathological injure of lung, while IL-27 can significantly inhibit it.