牦牛和柴达木黄牛的颈动脉体Ⅰ型细胞线粒体和电子致密核心囊泡的体视学比较

Stereologic Study on Mitochondria and Electron Dense-cored Vesicles of Carotid Body TypeⅠ Cells between Yak and Qaidam Yellow Cattle

作者旨在比较牦牛和柴达木黄牛颈动脉体(CB)Ⅰ型细胞线粒体和电子致密核心囊泡(EDCV)的体视学特征。选择海拔3 200m地区健康成年牦牛和柴达木黄牛各5头,采用透射电镜技术观察CB的超微结构,并运用立体定量方法比较牦牛和柴达木黄牛颈动脉体Ⅰ型细胞线粒体和EDCV的体密度(Vν)、面密度(Sν)、面数密度(NA)、比表面(δ)。结果表明,牦牛和柴达木黄牛的颈动脉体实质细胞主要由Ⅰ型细胞和Ⅱ型细胞组成,颈动脉体Ⅰ型细胞细胞质中含有大量的线粒体和特征性的电子致密核心囊泡。牦牛Ⅰ型细胞线粒体的Vν值大于柴达木黄牛,但差异不显著(P>0.05);牦牛S_v、N_A、δ值均小于柴达木黄牛,其中Sν、δ值在两组间差异均不显著(P>0.05),NA在两组间差异显著(P<0.05);牦牛Ⅰ型细胞质内EDCV的Vν值小于柴达木黄牛(P>0.05);牦牛Sν、NA和δ值均大于柴达木黄牛(P>0.05)。结果提示这种结构特征可能使牦牛对环境低氧的感知比生活在相同海拔高度的柴达木黄牛迟钝。

The aim of the present study was to compare the stereology characteristics of mitochondria and electron dense-cored vesicles（EDCVs）of carotid body（CB）type Ⅰ cells between yaks and Qaidam yellow cattle.In this study,the ultrastructures of CB in health adult yaks（n=5）and Qaidam yellow cattle（n=5）at altitude of 3 200 m were observed by electron transmission microscopy technique,and the volume density（Vν）,surface density（Sν）,numerical density on area（NA）and specific surface（δ）of mitochondria and EDCV of CB type Ⅰ cells between yaks and Qaidam yellow cattle were compared by stereology.The results showed that the CB parenchyma cells were composed of type Ⅰ cells with many mitochondria and EDCVs in the cytoplasm and typeⅡ cells in yaks and Qaidam yellow cattle,The Vνof mitochondria of CB typeⅠcells in yaks were greater than that of in Qaidam yellow cattle（P 〈0.05）,the Sν,NA,δwere less than that of in Qaidam yellow cattles,with no significant difference in Sνandδbetween yaks and Qaidam yellow cattle（P 〈0.05）and significant difference in NAbetween 2gropes（P 〈0.05）;The Vνof EDCV of CB typeⅠ cells in yaks were less than that of in Qaidam yellow cattle（P 〈0.05）,andthe Sν,NA,δwere slightly greater than that of in Qaidam yellow cattle（P 〈0.05）,respectively.The results suggest that the structure characteristics may make the yak was more insensive to perceive hypoxia environment than the Qaidam yellow cattle.