灰毛豆中异黄酮类、鱼藤酮类及coumestan类化合物分析

Analysis of Isoflavonids,Rotenoids and Coumestans From Tephrosia purpurea

目的:探讨灰毛豆Tephrosia purpurea枝叶部位的化学成分;丰富灰毛豆属植物的化学成分数据库。方法:采用多种色谱分离手段和现代波谱分析方法以及通过对照文献,深入系统地对灰毛豆T.purpurea的枝叶部分进行了化学成分分离提取及结构鉴定。结果:从灰毛豆T.purpurea中共分离鉴定出10个异黄酮类化合物,分别为12a-dehydro-6-hydroxysumatrol(1),elongatin(2),tephcalostan C(3),7,4'-dihydroxy-3',5'-dimethoxyisoflavone(4),4'-demethyltoxicarol isoflavone(5),tephcalostan B(6),5,7-di-O-prenylbiochanin A(7),tephcalostan D(8),tephcalostan(9),6a,12a-dehydro-2,3,6-trimethoxy-8-(3',3'-dimethylallyl)-9,11-dihydroxyrotenone(10),3,4,8,9-dimethylenedioxypterocarpan(11),12a-hydroxy-β-toxicarol(12)。结论:除化合物4外,其他11个化合物均为首次从该植物中分离得到。

Objective： There is a rich resource of Tephrosia in China. This paper is to investigate the chemical components from the genius of Tephrosia purpurea. Method： The chemical compounds of T. purpurea branches and leaves were isolated and extracted, and their structures were identified by using several chromatographic separation methods, modern spectrum analysis method and literature comparison. Result： Twelve isoflavones were obtained from the branches and leaves of T. purpurea and identified as follows： 12a-dehydro-6- hydroxysumatrol （ 1 ）, elongatin （2）, tephcalostan C （3）, 7, 4＇-dihydroxy-3＇, 5＇-dimethoxyisoflavone （4）, 4＇- demethyhoxicarol isoflavone （5）, tephcalostan B （6）, 5, 7-di-O-prenylbiochanin A （7）, tephcalostan D （8）, tephcalostan （ 9 ）, 6a, 12a-dehydro-2, 3, 6-trimethoxy-8-（ 3＇, 3＇-dimethylallyl ）-9, ll-dihydroxyrotenone （10）, 3, 4, 8, 9-dimethylenedioxypterocarpan （11）, 12a-hydroxy-β-toxicarol （12）. Conclusion ： All the other 11 compounds except compound 4 were obtained from this plant for the first time.