巴豆油中佛波醇类成分的HPLC指纹图谱

HPLC Fingerprint of Phorbols in Crotonis Fructus Oil

目的:建立巴豆油中佛波醇类的HPLC指纹图谱,为巴豆油的质量评价提供依据。方法:采用RP-HPLC,测定了10批巴豆样品。色谱条件为Thermo Hypersil Gold C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相水-乙腈梯度洗脱,流速1.0m L·min^(-1),柱温30℃,检测波长235 nm。结果:在建立的巴豆油中佛波醇类指纹图谱中,10批样品HPLC指纹图谱相似度在0.884~0.991,确立了12个共有峰的共有模式。结论:该方法简便、准确、重复性好,为评价巴豆油定性鉴别及内在质量提供了科学依据。

Objective： To establish the HPLC Phorbols part in Crotonis Fructus oil, and offer the evidence for quality evaluation of Crotonis Fructus oil accordingly. Method： To measure ten batches of Crotonis Fructus by using RP-HPLC. The separation condition was： Thermo Hypersil Gold C18 chromatographic column （4.6 mm×250 mm,5μm） , with water as mobile phase A, and acetonitrile as mobile phase B in gradient mode, and the flow rate is 1.0 mL·min-1, column temperature was set at 30℃, detection wavelength was at 235 rim. Result： The common mode of the HPLC fingerprints were setup by ensuring twelve common peaks in the chromatograms. The similar degrees to the ten batches were between 0. 884-0. 991. Conclusion： The method is simple, accurate and have a good repeatability. And the quality of Crotonis Fructus oil can be controlled effectively by the HPLC fingerprint.