RP-HPLC同时测定不同产地路边青药材中4种活性成分的含量

Simultaneous Determination of Four Constituents in Geum aleppicum by RP-HPLC

目的:建立反相高效液相色谱法同时测定路边青药材中咖啡酸、槲皮素-3-O-葡萄糖苷、山柰酚-3-O-葡萄糖苷、山柰酚含量的方法。方法:采用Agilent C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.5%甲酸水溶液梯度洗脱,流速0.8 m L·min^(-1),柱温25℃,检测波长340 nm。结果:咖啡酸、槲皮素-3-O-葡萄糖苷、山柰酚-3-O-葡萄糖苷、山柰酚分别在0.045~1.130,0.095~2.370,0.105~2.635,0.041~1.030μg线性关系良好,平均加样回收率(n=6)分别为99.0%,98.5%,97.7%,100.7%,RSD分别为1.0%,0.9%,1.0%,1.6%。结论:该方法简便、快捷,重复性好,可用于路边青药材的质量控制。

Objective： To establish a method for simultaneously determining caffeic acid, quercetin-3- O-glucoside, kaempferol-3-O-glucoside and kaempferol in Geum aleppicum by RP-HPLC. Method： The quantitative analysis of G. aleppicum was carried out on Agilent Cls column （4.6 mm×250 mm,5μm） , mobile phase was acetonitrile-0.5% formic acid with gradient elution, flow rate was 0. 8 mL·min -1, columntemperature was maintained at 25℃ , and detection wavelengrt was 340 nm. Result： The linear relationships of caffeic acid, quercetin-3-O-glucoside, kaempferol-3-O-glucoside and kaempferol fell within the ranges of 0.045-1. 130, 0. 095-2. 370, 0. 105-2. 635, 0. 041-1. 030 Ixg, respectively. Their average recoveries （ n = 6） were 99.0% , 98.5%, 97.7%, 100.7% with the relative standard deviations （RSDs） of 1.0%, 0.9%, 1.0%, 1.6%, respectively. Conclusion： This method is simple, accurate, sensitive, which can be used for quality control of G. aleppicum.