右归胶囊防治去卵巢大鼠骨质疏松的作用

Effect of Yougui Capsule on Osteoporosis in Ovariectomized Rats

目的:观察右归胶囊防治去卵巢大鼠骨质疏松的作用,并探讨其相关的作用机制。方法:SPF级SD大鼠105只,其中15只大鼠作为假手术组,其余大鼠采用去卵巢大鼠复制骨质疏松模型,取造模后雌性大鼠随机分为模型组,右归胶囊低、中、高剂量组(0.71,1.43,2.85 g·kg^(-1)),雌激素组(己烯雌酚,0.02 mg·kg^(-1))和仙灵骨葆胶囊组(0.25 g·kg^(-1)),每组15只,于术后第7天开始ig给药,连续给药6个月。于末次给药后24 h大鼠眼眶采血,测定血清钙(Ca),磷(P),碱性磷酸酶(ALP),酸性磷酸酶(ACP)。完整取出大鼠左右股骨,用双能X射线吸收法骨密度仪测大鼠左股骨骨密度(BMD)和骨矿质(BMC)。用WDW型电子万能实验机测大鼠右股骨力学性能指标挠度、最大力和抗弯强度。结果:与假手术组比较,模型组大鼠血清Ca,P,ALP及ACP水平明显升高,大鼠BMD及BMC含量明显降低,大鼠右股骨挠度、最大力和抗弯强度明显降低(P<0.05,P<0.01);与模型组比较,右归胶囊低、中、高剂量组均能明显降低去势大鼠血清Ca,P,ALP及ACP水平,明显提高去势大鼠BMD及BMC含量,明显升高右股骨挠度、最大力和抗弯强度(P<0.05,P<0.01)。结论:右归胶囊能改善去势大鼠生物力学指标,并能够延缓大鼠骨量丢失,提示其具有预防骨质疏松症作用。

Objective： To observe the effects of Yougui capsule on osteoporosis in ovariectomized rats. Method： A total of 105 SPF-grade SD rats were selected, in which 15 were used in the sham-operated group, and the remaining were ovariectomized to reproduce the model of osteoporosis. The ovariectomized rats were divided into model group, Yougui capsule low, medium and high-dose groups （0.71, 1.43, 2.85 g·kg-1）, estrogen group （diethylstilbestrol, 0.02 mg·kg-1） and Xianling Gubao capsule group （0.25 g·kg-1）, with 15 rats in each group. The rats were given the corresponding drugs on the 7th day after surgery for six months. Orbital blood in rats was collected to determine Ca, P, alkaline phosphatase （ALP）, acid phosphatase （ACP） in serum. Right and left thighbones of the rats were collected to determine bone mineral density （BMD）, bone mineral （BMC） with dual energy X-ray absorptiometry. WDW electronic universal testing machine was used to determine mechanical performance indexes of the right thighbone, such as deflection, maximum force and bending strength. Result： Compared with the sham-operated group, the model group showed significant increases in Ca, P, ALP, ACP levels in serum, and decreases in BMD, BMC and bone biomechanical indexes （P 〈0.05, P 〈0.01 ）; compared with the model group, Yougui capsule groups can significantly decrease Ca, P, ALP, ACP level in serum, and increase BMD, BMC and bone biomechanical indexes （P 〈0.05, P 〈0.01 ）. Conclusion： Yougui capsule can improve biomechanical indexes of ovariectomized rats, and delay the rat bone mass loss, suggesting that Yougui capsule has preventive effect on osteoporosis.