不同分级与分期膀胱癌人微小核糖核酸3658和人源性长寿保障基因Ⅱ型表达的变化

Expression of hsa-miR-3658 and LASS2 genes in bladder cancer of different pathological grades and clinical stages

目的检测人微小核糖核酸3658(miR-3658)和人源性长寿保障基因Ⅱ型(LASS2)在不同病理分级、临床分期膀胱癌组织中的表达情况。方法通过实时荧光定量PCR检测96对新鲜膀胱癌组织(膀胱癌组)和癌旁正常组织(癌旁组)中miR-3658和LASS2基因的表达情况;采用免疫组化法检测LASS2的蛋白表达。结果采用2^(-ΔΔCt)的相对表达量分析,膀胱癌组的miR-3658表达水平显著高于癌旁组,而膀胱癌组LASS2的表达水平低于癌旁组(P<0.01)。不同病理分级、临床分期膀胱癌组织之间,miR-3658和LASS2基因均呈差异性表达。与高分化膀胱癌相比,低分化膀胱癌miR-3658呈高表达,LASS2呈低表达(P<0.01)。随着肿瘤浸润深度及临床分期的增加,miR-3658表达水平呈递增趋势,而LASS2则逐渐下降(P<0.01)。免疫组化结果显示,膀胱癌组LASS2蛋白阳性表达率低于癌旁组,且随着肿瘤病理分级、浸润深度、分期程度的增加,其阳性表达率逐渐降低(P<0.05)。结论 miR-3658的高表达及LASS2的低表达可能参与膀胱癌的发生发展过程,并与其恶性程度相关。

Objective To investigate the expression of hsa - microRNA - 3658 （ miR - 3658） and Homo sapiens longevity assurance homologue2 （LASS2） genes in bladder cancer tissues with different pathological grades and clinical stages. Methods The expression of miR - 3658 and LASS2 mRNA in 96 paired tissues, which acquired from bladder cancer and normal epithelium tissues beside the carcinoma, was assessed using quantitative reverse transcription PCR. The technology of immunohistochemistry stain （IHC） was also performed to detect the expression of LASS2 in these tissues. Results According to 2-ΔΔCt, Method, miR - 3658 expression was significantly higher in bladder cancer tissues than adja- cent tissues, as the LASS2 expression was significantly lower in bladder cancer tissues （ P 〈 0. 01 ）. Both the expression of miR - 3658 and LASS2 genes were different in bladder cancer tissues with different pathological grades and clinical stages. Compared to the highly differentiated bladder cancer tissues, poorly differentiated tissues presented with significantly up - regulated miR- 3658 accompanied and down- regulated LASS2 gene （P 〈 0. 01 ）. With increasing infiltration depth and clinical stages, the expression of miR - 3658 was gradually increased, with reduction in the expression of LASS2 gene. At protein level, the LASS2 positive rate was significantly lower in bladder cancer tissues than adjacent tissues （P 〈 0. 01 ） ; as significantly lower LASS2 expression was observed in higher pathological grade, infiltration depth and clinical stage car- cinoma tissues （P 〈 0. 05）. Conclusion LASS2 and miR - 3658 expression may be involved in the development and progression of bladder cancer, and may be prognostic indicators for this cancer.